Staff at EMBL

Dr. Imre Berger

Group Leader
Berger Group Joint appointment: Genome Biology Unit

EMBL Grenoble, BP 181, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France

Biography

PhD 1995, MIT Cambridge and Leibniz University, Hannover.
Postdoctoral research at MIT and the Institute of Molecular Biology and Biophysics (IMB), ETH Zürich.
Habilitation 2005, ETH.
Group leader at IMB since 2005.
Group leader at EMBL Grenoble since 2007.

Publications

Structural basis of signal sequence surveillance and selection by the SRP-FtsY complex. von Loeffelholz, O., Knoops, K., Ariosa, A., Zhang, X., Karuppasamy, M., Huard, K., Schoehn, G., Berger, I., Shan, S.O. & Schaffitzel, C. Nat Struct Mol Biol. 2013 May;20(5):604-10. doi: 10.1038/nsmb.2546. Epub 2013 Apr7. PubMed

Baculovirus expression: tackling the complexity challenge. Barford, D., Takagi, Y., Schultz, P. & Berger, I. Curr Opin Struct Biol. 2013 Apr 26. pii: S0959-440X(13)00052-3. doi:10.1016/j.sbi.2013.03.009. PubMed

The architecture of human general transcription factor TFIID core complex. Bieniossek, C., Papai, G., Schaffitzel, C., Garzoni, F., Chaillet, M., Scheer, E., Papadopoulos, P., Tora, L., Schultz, P. & Berger, I. Nature. 2013 Jan 31;493(7434):699-702. doi: 10.1038/nature11791. Epub 2013 Jan 6. PubMed

Gene gymnastics: Synthetic biology for baculovirus expression vector system engineering. Vijayachandran, L.S., Thimiri Govinda Raj, D.B., Edelweiss, E., Gupta, K., Maier, J., Gordeliy, V., Fitzgerald, D.J. & Berger, I. Bioengineered. 2013 Jan 17;4(5). PubMed

MultiBac complexomics. Trowitzsch, S., Palmberger, D., Fitzgerald, D., Takagi, Y. & Berger, I. Expert Rev Proteomics. 2012 Aug;9(4):363-73. PubMed

MultiBac turns sweet. Palmberger, D., Klausberger, M., Berger, I. & Grabherr, R. Bioengineered. 2012 Mar 1;4(2). PubMed

MultiBac: expanding the research toolbox for multiprotein complexes. Bieniossek, C., Imasaki, T., Takagi, Y. & Berger, I. Trends Biochem Sci. 2012 Feb;37(2):49-57. doi: 10.1016/j.tibs.2011.10.005. Epub2011 Dec 7. PubMed

SweetBac: A New Approach for the Production of Mammalianised Glycoproteins in Insect Cells. Palmberger, D., Wilson, I.B., Berger, I., Grabherr, R. & Rendic, D. PLoS One. 2012;7(4):e34226. Epub 2012 Apr 2. PubMed

Light it up: Highly efficient multigene delivery in mammalian cells. Trowitzsch, S., Klumpp, M., Thoma, R., Carralot, J.P. & Berger, I. Bioessays. 2011 Dec;33(12):946-55. doi: 10.1002/bies.201100109. Epub 2011Oct 17. PubMed

Robots, pipelines, polyproteins: enabling multiprotein expression in prokaryotic and eukaryotic cells. Vijayachandran, L.S., Viola, C., Garzoni, F., Trowitzsch, S., Bieniossek, C., Chaillet, M., Schaffitzel, C., Busso, D., Romier, C., Poterszman, A., Richmond, T.J. & Berger, I. J Struct Biol. 2011 Aug;175(2):198-208. doi: 10.1016/j.jsb.2011.03.007. Epub 2011Mar 17. PubMed

Structural insights into transcription complexes. Berger, I., Blanco, A.G., Boelens, R., Cavarelli, J., Coll, M., Folkers, G.E., Nie, Y., Pogenberg, V., Schultz, P., Wilmanns, M., Moras, D. & Poterszman, A. J Struct Biol. 2011 Aug;175(2):135-46. doi: 10.1016/j.jsb.2011.04.015. Epub 2011May 6. PubMed

Architecture of the Mediator head module. Imasaki, T., Calero, G., Cai, G., Tsai, K.L., Yamada, K., Cardelli, F., Erdjument-Bromage, H., Tempst, P., Berger, I., Kornberg, G.L., Asturias, F.J., Kornberg, R.D. & Takagi, Y. Nature. 2011 Jul 3;475(7355):240-3. doi: 10.1038/nature10162. PubMed

Recombinant Heptameric Coatomer Complexes: Novel Tools to Study Isoform-Specific Functions. Sahlmuller, M.C., Strating, J.R., Beck, R., Eckert, P., Popoff, V., Haag, M., Hellwig, A., Berger, I., Brugger, B. & Wieland, F.T. Traffic. 2011 Jun;12(6):682-692. doi: 10.1111/j.1600-0854.2011.01177.x.Epub 2011 Mar 15. PubMed

Baculovirus Expression Strategies for Protein Complex Production Vijayachandran, L.S., Viola, C. & Berger, I. Encyclopedia of Life Sciences (ELS) /, John Wiley & Sons,Ltd:
Chichester
DOI: 10.1002/ 9780470015902.a0023180 (2011).

A plasmid-based multigene expression system for mammalian cells. Kriz, A., Schmid, K., Baumgartner, N., Ziegler, U., Berger, I., Ballmer-Hofer, K. & Berger, P. Nat Commun. 2010 Nov;1(8):120. PubMed

New baculovirus expression tools for recombinant protein complex production. Trowitzsch, S., Bieniossek, C., Nie, Y., Garzoni, F. & Berger, I. J Struct Biol. 2010 Oct;172(1):45-54. doi: 10.1016/j.jsb.2010.02.010. Epub 2010Feb 21. PubMed

Getting a grip on complexes. Nie, Y., Viola, C., Bieniossek, C., Trowitzsch, S., Vijay-Achandran, L.S., Chaillet, M., Garzoni, F. & Berger, I. Curr Genomics. 2009 Dec;10(8):558-72. PubMed

ACEMBLing multigene expression constructs by recombineering. Nie, Y., Bieniossek, C., Frey, D., Olieric, N., Schaffitzel, C., Steinmetz, M.O. & Berger, I. Nat Protocols, doi: 10.1038/nprot.2009.104

Automated unrestricted multigene recombineering for multiprotein complex production. Bieniossek, C., Nie, Y., Frey, D., Olieric, N., Schaffitzel, C., Collinson, I., Romier, C., Berger, P., Richmond, T.J., Steinmetz, M.O. & Berger, I. Nat Methods. 2009 Jun;6(6):447-50. Epub 2009 May 3. PubMed

Towards eukaryotic structural complexomics. Bieniossek, C. & Berger, I. J Struct Funct Genomics. 2009 Mar;10(1):37-46. Epub 2008 Nov 14. PubMed

MultiBac: multigene baculovirus-based eukaryotic protein complex production. Bieniossek, C., Richmond, T.J. & Berger, I. Curr Protoc Protein Sci. 2008 Feb;Chapter 5:Unit 5.20. PubMed

Multiprotein expression strategy for structural biology of eukaryotic complexes. Fitzgerald, D.J., Schaffitzel, C., Berger, P., Wellinger, R., Bieniossek, C., Richmond, T.J. & Berger, I. Structure. 2007 Mar;15(3):275-9. PubMed

Nucleic Acid Science - The Excitement of Discovery. Annual Symposium of the Chemical Society Zurich. Berger, I., Schaffitzel, C. & Bieniossek, C. Chimia 2007 61 837-841

Protein complex expression by using multigene baculoviral vectors. Fitzgerald, D.J., Berger, P., Schaffitzel, C., Yamada, K., Richmond, T.J. & Berger, I. Nat Methods. 2006 Dec;3(12):1021-32. PubMed

Structure of the E. coli signal recognition particle bound to a translating ribosome. Schaffitzel, C., Oswald, M., Berger, I., Ishikawa, T., Abrahams, J.P., Koerten, H.K., Koning, R.I. & Ban, N. Nature. 2006 Nov 23;444(7118):503-6. Epub 2006 Oct 29. PubMed

Multi-level regulation of myotubularin-related protein-2 phosphatase activity by myotubularin-related protein-13/set-binding factor-2. Berger, P., Berger, I., Schaffitzel, C., Tersar, K., Volkmer, B. & Suter, U. Hum Mol Genet. 2006 Feb 15;15(4):569-79. Epub 2006 Jan 6. PubMed

Syntheses of 4'-thioribonucleosides and thermodynamic stability and crystal structure of RNA oligomers with incorporated 4'-thiocytosine. Haeberli, P., Berger, I., Pallan, P.S. & Egli, M. Nucleic Acids Res. 2005 Jul 18;33(13):3965-75. Print 2005. PubMed

Baculovirus expression system for heterologous multiprotein complexes. Berger, I., Fitzgerald, D.J. & Richmond, T.J. Nat Biotechnol. 2004 Dec;22(12):1583-7. Epub 2004 Nov 28. PubMed

Reaction cycle of the yeast Isw2 chromatin remodeling complex. Fitzgerald, D.J., DeLuca, C., Berger, I., Gaillard, H., Sigrist, R., Schimmele, K. & Richmond, T.J. EMBO J. 2004 Oct 1;23(19):3836-43. Epub 2004 Sep 9. PubMed

Membrane association of myotubularin-related protein 2 is mediated by a pleckstrin homology-GRAM domain and a coiled-coil dimerization module. Berger, P., Schaffitzel, C., Berger, I., Ban, N. & Suter, U. Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12177-82. Epub 2003 Oct 6. PubMed

Direct interaction of Ca2+/calmodulin inhibits histone deacetylase 5 repressor core binding to myocyte enhancer factor 2. Berger, I., Bieniossek, C., Schaffitzel, C., Hassler, M., Santelli, E. & Richmond, T.J. J Biol Chem. 2003 May 16;278(20):17625-35. Epub 2003 Mar 6. PubMed

In vitro generated antibodies specific for telomeric guanine-quadruplex DNA react with Stylonychia lemnae macronuclei. Schaffitzel, C., Berger, I., Postberg, J., Hanes, J., Lipps, H.J. & Pluckthun, A. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8572-7. Epub 2001 Jul 3. PubMed

Structural dynamics and cation interactions of DNA quadruplex molecules containing mixed guanine/cytosine quartets revealed by large-scale MD simulations. Spackova, N., Berger, I. & Sponer, J. J Am Chem Soc. 2001 Apr 11;123(14):3295-307. PubMed

Inter-strand C-H...O hydrogen bonds stabilizing four-stranded intercalated molecules: stereoelectronic effects of O4' in cytosine-rich DNA. Berger, I., Egli, M. & Rich, A. Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12116-21. PubMed

Crystal structure of the T4 regA translational regulator protein at 1.9 A resolution. Kang, C., Chan, R., Berger, I., Lockshin, C., Green, L., Gold, L. & Rich, A. Science. 1995 May 26;268(5214):1170-3. PubMed