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Staff at EMBL


Dr. Imre Berger

Group Leader
Berger Group Joint appointment: Genome Biology Unit

EMBL Grenoble, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France

E-mail: send a mail


PhD 1995, MIT Cambridge and Leibniz University, Hannover.
Postdoctoral research at MIT and the Institute of Molecular Biology and Biophysics (IMB), ETH Zürich.
Habilitation 2005, ETH.
Group leader at IMB since 2005.
Group leader at EMBL Grenoble since 2007.


Total: 44 publication(s)


Reich S, Guilligay D, Pflug A, Malet H, Berger I, Crépin T, Hart D, Lunardi T, Nanao M, Ruigrok RW, Cusack S. (2014)
Structural insight into cap-snatching and RNA synthesis by influenza polymerase.
Nature doi:10.1038/nature14009
Europe PMC | doi

Schulze RJ, Komar J, Botte M, Allen WJ, Whitehouse S, Gold VA, Lycklama A Nijeholt JA, Huard K, Berger I, Schaffitzel C, Collinson I. (2014)
Membrane protein insertion and proton-motive-force-dependent secretion through the bacterial holo-translocon SecYEG-SecDF-YajC-YidC
Proc. Natl. Acad. Sci. U.S.A. 111(13):4844-4849. doi:10.1073/pnas.1315901111
Europe PMC | doi

Kandiah E, Trowitzsch S, Gupta K, Haffke M, Berger I. (2014)
More pieces to the puzzle: recent structural insights into class II transcription initiation
Curr. Opin. Struct. Biol. 24C:91-97. doi:10.1016/
Europe PMC | doi

Nie Y, Bellon-Echeverria I, Trowitzsch S, Bieniossek C, Berger I. (2014)
Multiprotein complex production in insect cells by using polyproteins
Methods Mol. Biol. 1091:131-141. doi:10.1007/978-1-62703-691-7_8
Europe PMC | doi

Raj DB, Vijayachandran LS, Berger I. (2014)
OmniBac: universal multigene transfer plasmids for baculovirus expression vector systems
Methods Mol. Biol. 1091:123-130. doi:10.1007/978-1-62703-691-7_7
Europe PMC | doi


Berger I, Chaillet M, Garzoni F, Yau-Rose S, Zoro B. (2013)
High-Throughput Screening of Multiple Protein Complexes
AM LAB 45(8):32-35.

Barford D, Takagi Y, Schultz P, Berger I. (2013)
Baculovirus expression: tackling the complexity challenge
Curr. Opin. Struct. Biol. 23(3):357-364. doi:10.1016/
Europe PMC | doi

Berger I, Mayr LM. (2013)
Protein production for structural biology: new solutions to new challenges
Curr. Opin. Struct. Biol. 23(3):317-318. doi:10.1016/
Europe PMC | doi

von Loeffelholz O, Knoops K, Ariosa A, Zhang X, Karuppasamy M, Huard K, Schoehn G, Berger I, Shan SO, Schaffitzel C. (2013)
Structural basis of signal sequence surveillance and selection by the SRP-FtsY complex
Nat. Struct. Mol. Biol. 20(5):604-610. doi:10.1038/nsmb.2546
Europe PMC | doi

Vijayachandran LS, Thimiri Govinda Raj DB, Edelweiss E, Gupta K, Maier J, Gordeliy V, Fitzgerald DJ, Berger I. (2013)
Gene gymnastics: Synthetic biology for baculovirus expression vector system engineering
Bioengineered 4(5):279-287. doi:10.4161/bioe.22966
Europe PMC | doi

Palmberger D, Klausberger M, Berger I, Grabherr R. (2013)
MultiBac turns sweet
Bioengineered 4(2):78-83. doi:10.4161/bioe.22327
Europe PMC | doi

Haffke M, Viola C, Nie Y, Berger I. (2013)
Tandem recombineering by SLIC cloning and Cre-LoxP fusion to generate multigene expression constructs for protein complex research
Methods Mol. Biol. 1073:131-140. doi:10.1007/978-1-62703-625-2_11
Europe PMC | doi

Bieniossek C, Papai G, Schaffitzel C, Garzoni F, Chaillet M, Scheer E, Papadopoulos P, Tora L, Schultz P, Berger I. (2013)
The architecture of human general transcription factor TFIID core complex
Nature 493(7434):699-702. doi:10.1038/nature11791
Europe PMC | doi

Berger I, Garzoni F, Chaillet M, Haffke M, Gupta K, Aubert A. (2013)
The multiBac protein complex production platform at the EMBL
J Vis Exp (77):e50159. doi:10.3791/50159
Europe PMC | doi


Trowitzsch S, Palmberger D, Fitzgerald D, Takagi Y, Berger I. (2012)
MultiBac complexomics
Expert Rev Proteomics 9(4):363-373. doi:10.1586/epr.12.32
Europe PMC | doi

Bieniossek C, Imasaki T, Takagi Y, Berger I. (2012)
MultiBac: expanding the research toolbox for multiprotein complexes
Trends Biochem. Sci. 37(2):49-57. doi:10.1016/j.tibs.2011.10.005
Europe PMC | doi

Palmberger D, Wilson IB, Berger I, Grabherr R, Rendic D. (2012)
SweetBac: a new approach for the production of mammalianised glycoproteins in insect cells
PLoS ONE 7(4):e34226. doi:10.1371/journal.pone.0034226
Europe PMC | doi


Trowitzsch S, Klumpp M, Thoma R, Carralot JP, Berger I. (2011)
Light it up: highly efficient multigene delivery in mammalian cells
Bioessays 33(12):946-955. doi:10.1002/bies.201100109
Europe PMC | doi

Vijayachandran LS, Viola C, Garzoni F, Trowitzsch S, Bieniossek C, Chaillet M, Schaffitzel C, Busso D, Romier C, Poterszman A, Richmond TJ, Berger I. (2011)
Robots, pipelines, polyproteins: enabling multiprotein expression in prokaryotic and eukaryotic cells
J. Struct. Biol. 175(2):198-208. doi:10.1016/j.jsb.2011.03.007
Europe PMC | doi

Berger I, Blanco AG, Boelens R, Cavarelli J, Coll M, Folkers GE, Nie Y, Pogenberg V, Schultz P, Wilmanns M, Moras D, Poterszman A. (2011)
Structural insights into transcription complexes
J. Struct. Biol. 175(2):135-146. doi:10.1016/j.jsb.2011.04.015
Europe PMC | doi

Imasaki T, Calero G, Cai G, Tsai KL, Yamada K, Cardelli F, Erdjument-Bromage H, Tempst P, Berger I, Kornberg GL, Asturias FJ, Kornberg RD, Takagi Y. (2011)
Architecture of the Mediator head module
Nature 475(7355):240-243. doi:10.1038/nature10162
Europe PMC | doi

Sahlmüller MC, Strating JR, Beck R, Eckert P, Popoff V, Haag M, Hellwig A, Berger I, Brügger B, Wieland FT. (2011)
Recombinant heptameric coatomer complexes: novel tools to study isoform-specific functions
Traffic 12(6):682-692. doi:10.1111/j.1600-0854.2011.01177.x
Europe PMC | doi

Vijayachandran LS, Viola C, Berger I. (2011)
Baculovirus expression strategies for protein complex production
In: (various eds.), Encyclopedia of Life Sciences (eLS). John Wiley & Sons, Ltd.doi:10.1002/9780470015902.a0023180
Europe PMC | doi


Trowitzsch S, Bieniossek C, Nie Y, Garzoni F, Berger I. (2010)
New baculovirus expression tools for recombinant protein complex production
J. Struct. Biol. 172(1):45-54. doi:10.1016/j.jsb.2010.02.010
Europe PMC | doi

Kriz A, Schmid K, Baumgartner N, Ziegler U, Berger I, Ballmer-Hofer K, Berger P. (2010)
A plasmid-based multigene expression system for mammalian cells
Nat Commun 1:120. doi:10.1038/ncomms1120
Europe PMC | doi


Nie Y, Viola C, Bieniossek C, Trowitzsch S, Vijay-Achandran LS, Chaillet M, Garzoni F, Berger I. (2009)
Getting a grip on complexes
Curr. Genomics 10(8):558-572. doi:10.2174/138920209789503923
Europe PMC | doi

Bieniossek C, Nie Y, Frey D, Olieric N, Schaffitzel C, Collinson I, Romier C, Berger P, Richmond TJ, Steinmetz MO, Berger I. (2009)
Automated unrestricted multigene recombineering for multiprotein complex production
Nat. Methods 6(6):447-450. doi:10.1038/nmeth.1326
Europe PMC | doi

Nie Y, Bieniossek C, Frey D, Olieric N, Schaffitzel C. (2009)
ACEMBLing multigene expression vectors by recombineering
Protocol Exchange doi:10.1038/nprot.2009.104
Europe PMC | doi

Bieniossek C, Berger I. (2009)
Towards eukaryotic structural complexomics
J. Struct. Funct. Genomics 10(1):37-46. doi:10.1007/s10969-008-9047-6
Europe PMC | doi


Bieniossek C, Richmond TJ, Berger I. (2008)
MultiBac: multigene baculovirus-based eukaryotic protein complex production
Curr Protoc Protein Sci Chapter 5:Unit 5.20. doi:10.1002/0471140864.ps0520s51
Europe PMC | doi


Fitzgerald DJ, Schaffitzel C, Berger P, Wellinger R, Bieniossek C, Richmond TJ, Berger I. (2007)
Multiprotein expression strategy for structural biology of eukaryotic complexes
Structure 15(3):275-279. doi:10.1016/j.str.2007.01.016
Europe PMC | doi

Berger I, Schaffitzel C, Bieniossek C. (2007)
Nucleic Acid Science – The Excitement of Discovery: Annual Symposium of the Chemical Society Zurich CGZ, Zurich, October 26, 2007
Chimia (Aarau) 61(12):837-841.
Europe PMC


Fitzgerald DJ, Berger P, Schaffitzel C, Yamada K, Richmond TJ, Berger I. (2006)
Protein complex expression by using multigene baculoviral vectors
Nat. Methods 3(12):1021-1032. doi:10.1038/nmeth983
Europe PMC | doi

Schaffitzel C, Oswald M, Berger I, Ishikawa T, Abrahams JP, Koerten HK, Koning RI, Ban N. (2006)
Structure of the E. coli signal recognition particle bound to a translating ribosome
Nature 444(7118):503-506. doi:10.1038/nature05182
Europe PMC | doi

Berger P, Berger I, Schaffitzel C, Tersar K, Volkmer B, Suter U. (2006)
Multi-level regulation of myotubularin-related protein-2 phosphatase activity by myotubularin-related protein-13/set-binding factor-2
Hum. Mol. Genet. 15(4):569-579. doi:10.1093/hmg/ddi473
Europe PMC | doi


Haeberli P, Berger I, Pallan PS, Egli M. (2005)
Syntheses of 4'-thioribonucleosides and thermodynamic stability and crystal structure of RNA oligomers with incorporated 4'-thiocytosine
Nucleic Acids Res. 33(13):3965-3975. doi:10.1093/nar/gki704
Europe PMC | doi


Berger I, Fitzgerald DJ, Richmond TJ. (2004)
Baculovirus expression system for heterologous multiprotein complexes
Nat. Biotechnol. 22(12):1583-1587. doi:10.1038/nbt1036
Europe PMC | doi

Fitzgerald DJ, DeLuca C, Berger I, Gaillard H, Sigrist R, Schimmele K, Richmond TJ. (2004)
Reaction cycle of the yeast Isw2 chromatin remodeling complex
EMBO J. 23(19):3836-3843. doi:10.1038/sj.emboj.7600364
Europe PMC | doi


Berger P, Schaffitzel C, Berger I, Ban N, Suter U. (2003)
Membrane association of myotubularin-related protein 2 is mediated by a pleckstrin homology-GRAM domain and a coiled-coil dimerization module
Proc. Natl. Acad. Sci. U.S.A. 100(21):12177-12182. doi:10.1073/pnas.2132732100
Europe PMC | doi

Berger I, Bieniossek C, Schaffitzel C, Hassler M, Santelli E, Richmond TJ. (2003)
Direct interaction of Ca2+/calmodulin inhibits histone deacetylase 5 repressor core binding to myocyte enhancer factor 2
J. Biol. Chem. 278(20):17625-17635. doi:10.1074/jbc.m301646200
Europe PMC | doi


Schaffitzel C, Berger I, Postberg J, Hanes J, Lipps HJ, Plückthun A. (2001)
In vitro generated antibodies specific for telomeric guanine-quadruplex DNA react with Stylonychia lemnae macronuclei
Proc. Natl. Acad. Sci. U.S.A. 98(15):8572-8577. doi:10.1073/pnas.141229498
Europe PMC | doi

Spacková N, Berger I, Sponer J. (2001)
Structural dynamics and cation interactions of DNA quadruplex molecules containing mixed guanine/cytosine quartets revealed by large-scale MD simulations
J. Am. Chem. Soc. 123(14):3295-3307. doi:10.1021/ja002656y
Europe PMC | doi


Berger I, Egli M, Rich A. (1996)
Inter-strand C-H...O hydrogen bonds stabilizing four-stranded intercalated molecules: stereoelectronic effects of O4' in cytosine-rich DNA
Proc. Natl. Acad. Sci. U.S.A. 93(22):12116-12121. doi:10.1073/pnas.93.22.12116
Europe PMC | doi


Kang C, Chan R, Berger I, Lockshin C, Green L, Gold L, Rich A. (1995)
Crystal structure of the T4 regA translational regulator protein at 1.9 A resolution
Science 268(5214):1170-1173. doi:10.1126/science.7761833
Europe PMC | doi