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Staff at EMBL

Dr. Imre Berger

Group Leader
Berger Group Joint appointment: Genome Biology Unit

EMBL Grenoble, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France

E-mail: send a mail


PhD 1995, MIT Cambridge and Leibniz University, Hannover.
Postdoctoral research at MIT and the Institute of Molecular Biology and Biophysics (IMB), ETH Zürich.
Habilitation 2005, ETH.
Group leader at IMB since 2005.
Group leader at EMBL Grenoble since 2007.


Membrane protein insertion and proton-motive-force-dependent secretion through the bacterial holo-translocon SecYEG-SecDF-YajC-YidC. Schulze, R.J., Komar, J., Botte, M., Allen, W.J., Whitehouse, S., Gold, V.A., Lycklama A Nijeholt, J.A., Huard, K., Berger, I., Schaffitzel, C. & Collinson, I. Proc Natl Acad Sci U S A. 2014 Apr 1;111(13):4844-9. doi:10.1073/pnas.1315901111. Epub 2014 Feb 18. Europe PMC

More pieces to the puzzle: recent structural insights into class II transcription initiation. Kandiah, E., Trowitzsch, S., Gupta, K., Haffke, M. & Berger, I. Curr Opin Struct Biol. 2014 Jan 15;24C:91-97. doi: 10.1016/ Europe PMC

OmniBac: universal multigene transfer plasmids for baculovirus expression vector systems. Raj, D.B., Vijayachandran, L.S. & Berger, I. Methods Mol Biol. 2014;1091:123-30. doi: 10.1007/978-1-62703-691-7_7. Europe PMC

Multiprotein complex production in insect cells by using polyproteins. Nie, Y., Bellon-Echeverria, I., Trowitzsch, S., Bieniossek, C. & Berger, I. Methods Mol Biol. 2014;1091:131-41. doi: 10.1007/978-1-62703-691-7_8. Europe PMC

Gene gymnastics: Synthetic biology for baculovirus expression vector system engineering. Vijayachandran, L.S., Thimiri Govinda Raj, D.B., Edelweiss, E., Gupta, K., Maier, J., Gordeliy, V., Fitzgerald, D.J. & Berger, I. Bioengineered. 2013 Sep-Oct;4(5):279-87. doi: 10.4161/bioe.22966. Epub 2013 Jan17. Europe PMC

High-Throughput Screening of Multiple Protein Complexes. Berger, I., Chaillet, M., Garzoni, F., Yau-Rose, S. & Zoro, B. Amer. Laboratory. 2013 Sep;45(8): 32-35.

The multiBac protein complex production platform at the EMBL. Berger, I., Garzoni, F., Chaillet, M., Haffke, M., Gupta, K. & Aubert, A. J Vis Exp. 2013 Jul 11;(77):e50159. doi: 10.3791/50159. Europe PMC

Structural basis of signal sequence surveillance and selection by the SRP-FtsY complex. von Loeffelholz, O., Knoops, K., Ariosa, A., Zhang, X., Karuppasamy, M., Huard, K., Schoehn, G., Berger, I., Shan, S.O. & Schaffitzel, C. Nat Struct Mol Biol. 2013 May;20(5):604-10. doi: 10.1038/nsmb.2546. Epub 2013 Apr7. Europe PMC

Baculovirus expression: tackling the complexity challenge. Barford, D., Takagi, Y., Schultz, P. & Berger, I. Curr Opin Struct Biol. 2013 Apr 26. pii: S0959-440X(13)00052-3. doi:10.1016/ Europe PMC

The architecture of human general transcription factor TFIID core complex. Bieniossek, C., Papai, G., Schaffitzel, C., Garzoni, F., Chaillet, M., Scheer, E., Papadopoulos, P., Tora, L., Schultz, P. & Berger, I. Nature. 2013 Jan 31;493(7434):699-702. doi: 10.1038/nature11791. Epub 2013 Jan 6. Europe PMC

Protein production for structural biology: new solutions to new challenges. Berger, I. & Mayr, L.M. Curr Opin Struct Biol. 2013 Jun;23(3):317-8. doi: 10.1016/ Epub2013 Jun 2. Europe PMC

Tandem recombineering by SLIC cloning and Cre-LoxP fusion to generate multigene expression constructs for protein complex research. Haffke, M., Viola, C., Nie, Y. & Berger, I. Methods Mol Biol. 2013;1073:131-40. doi: 10.1007/978-1-62703-625-2_11. Europe PMC

MultiBac complexomics. Trowitzsch, S., Palmberger, D., Fitzgerald, D., Takagi, Y. & Berger, I. Expert Rev Proteomics. 2012 Aug;9(4):363-73. Europe PMC

MultiBac turns sweet. Palmberger, D., Klausberger, M., Berger, I. & Grabherr, R. Bioengineered. 2012 Mar 1;4(2). Europe PMC

MultiBac: expanding the research toolbox for multiprotein complexes. Bieniossek, C., Imasaki, T., Takagi, Y. & Berger, I. Trends Biochem Sci. 2012 Feb;37(2):49-57. doi: 10.1016/j.tibs.2011.10.005. Epub2011 Dec 7. Europe PMC

SweetBac: A New Approach for the Production of Mammalianised Glycoproteins in Insect Cells. Palmberger, D., Wilson, I.B., Berger, I., Grabherr, R. & Rendic, D. PLoS One. 2012;7(4):e34226. Epub 2012 Apr 2. Europe PMC

Light it up: Highly efficient multigene delivery in mammalian cells. Trowitzsch, S., Klumpp, M., Thoma, R., Carralot, J.P. & Berger, I. Bioessays. 2011 Dec;33(12):946-55. doi: 10.1002/bies.201100109. Epub 2011Oct 17. Europe PMC

Robots, pipelines, polyproteins: enabling multiprotein expression in prokaryotic and eukaryotic cells. Vijayachandran, L.S., Viola, C., Garzoni, F., Trowitzsch, S., Bieniossek, C., Chaillet, M., Schaffitzel, C., Busso, D., Romier, C., Poterszman, A., Richmond, T.J. & Berger, I. J Struct Biol. 2011 Aug;175(2):198-208. doi: 10.1016/j.jsb.2011.03.007. Epub 2011Mar 17. Europe PMC

Structural insights into transcription complexes. Berger, I., Blanco, A.G., Boelens, R., Cavarelli, J., Coll, M., Folkers, G.E., Nie, Y., Pogenberg, V., Schultz, P., Wilmanns, M., Moras, D. & Poterszman, A. J Struct Biol. 2011 Aug;175(2):135-46. doi: 10.1016/j.jsb.2011.04.015. Epub 2011May 6. Europe PMC

Architecture of the Mediator head module. Imasaki, T., Calero, G., Cai, G., Tsai, K.L., Yamada, K., Cardelli, F., Erdjument-Bromage, H., Tempst, P., Berger, I., Kornberg, G.L., Asturias, F.J., Kornberg, R.D. & Takagi, Y. Nature. 2011 Jul 3;475(7355):240-3. doi: 10.1038/nature10162. Europe PMC

Recombinant Heptameric Coatomer Complexes: Novel Tools to Study Isoform-Specific Functions. Sahlmuller, M.C., Strating, J.R., Beck, R., Eckert, P., Popoff, V., Haag, M., Hellwig, A., Berger, I., Brugger, B. & Wieland, F.T. Traffic. 2011 Jun;12(6):682-692. doi: 10.1111/j.1600-0854.2011.01177.x.Epub 2011 Mar 15. Europe PMC

Baculovirus Expression Strategies for Protein Complex Production Vijayachandran, L.S., Viola, C. & Berger, I. Encyclopedia of Life Sciences (ELS) /, John Wiley & Sons,Ltd:
DOI: 10.1002/9780470015902.a0023180 (2011).

A plasmid-based multigene expression system for mammalian cells. Kriz, A., Schmid, K., Baumgartner, N., Ziegler, U., Berger, I., Ballmer-Hofer, K. & Berger, P. Nat Commun. 2010 Nov;1(8):120. Europe PMC

New baculovirus expression tools for recombinant protein complex production. Trowitzsch, S., Bieniossek, C., Nie, Y., Garzoni, F. & Berger, I. J Struct Biol. 2010 Oct;172(1):45-54. doi: 10.1016/j.jsb.2010.02.010. Epub 2010Feb 21. Europe PMC

Getting a grip on complexes. Nie, Y., Viola, C., Bieniossek, C., Trowitzsch, S., Vijay-Achandran, L.S., Chaillet, M., Garzoni, F. & Berger, I. Curr Genomics. 2009 Dec;10(8):558-72. Europe PMC

ACEMBLing multigene expression constructs by recombineering. Nie, Y., Bieniossek, C., Frey, D., Olieric, N., Schaffitzel, C., Steinmetz, M.O. & Berger, I. Nat Protocols, doi: 10.1038/nprot.2009.104

Automated unrestricted multigene recombineering for multiprotein complex production. Bieniossek, C., Nie, Y., Frey, D., Olieric, N., Schaffitzel, C., Collinson, I., Romier, C., Berger, P., Richmond, T.J., Steinmetz, M.O. & Berger, I. Nat Methods. 2009 Jun;6(6):447-50. Epub 2009 May 3. Europe PMC

Towards eukaryotic structural complexomics. Bieniossek, C. & Berger, I. J Struct Funct Genomics. 2009 Mar;10(1):37-46. Epub 2008 Nov 14. Europe PMC

MultiBac: multigene baculovirus-based eukaryotic protein complex production. Bieniossek, C., Richmond, T.J. & Berger, I. Curr Protoc Protein Sci. 2008 Feb;Chapter 5:Unit 5.20. Europe PMC

Multiprotein expression strategy for structural biology of eukaryotic complexes. Fitzgerald, D.J., Schaffitzel, C., Berger, P., Wellinger, R., Bieniossek, C., Richmond, T.J. & Berger, I. Structure. 2007 Mar;15(3):275-9. Europe PMC

Nucleic Acid Science - The Excitement of Discovery. Annual Symposium of the Chemical Society Zurich. Berger, I., Schaffitzel, C. & Bieniossek, C. Chimia 2007 61 837-841

Protein complex expression by using multigene baculoviral vectors. Fitzgerald, D.J., Berger, P., Schaffitzel, C., Yamada, K., Richmond, T.J. & Berger, I. Nat Methods. 2006 Dec;3(12):1021-32. Europe PMC

Structure of the E. coli signal recognition particle bound to a translating ribosome. Schaffitzel, C., Oswald, M., Berger, I., Ishikawa, T., Abrahams, J.P., Koerten, H.K., Koning, R.I. & Ban, N. Nature. 2006 Nov 23;444(7118):503-6. Epub 2006 Oct 29. Europe PMC

Multi-level regulation of myotubularin-related protein-2 phosphatase activity by myotubularin-related protein-13/set-binding factor-2. Berger, P., Berger, I., Schaffitzel, C., Tersar, K., Volkmer, B. & Suter, U. Hum Mol Genet. 2006 Feb 15;15(4):569-79. Epub 2006 Jan 6. Europe PMC

Syntheses of 4'-thioribonucleosides and thermodynamic stability and crystal structure of RNA oligomers with incorporated 4'-thiocytosine. Haeberli, P., Berger, I., Pallan, P.S. & Egli, M. Nucleic Acids Res. 2005 Jul 18;33(13):3965-75. Print 2005. Europe PMC

Baculovirus expression system for heterologous multiprotein complexes. Berger, I., Fitzgerald, D.J. & Richmond, T.J. Nat Biotechnol. 2004 Dec;22(12):1583-7. Epub 2004 Nov 28. Europe PMC

Reaction cycle of the yeast Isw2 chromatin remodeling complex. Fitzgerald, D.J., DeLuca, C., Berger, I., Gaillard, H., Sigrist, R., Schimmele, K. & Richmond, T.J. EMBO J. 2004 Oct 1;23(19):3836-43. Epub 2004 Sep 9. Europe PMC

Membrane association of myotubularin-related protein 2 is mediated by a pleckstrin homology-GRAM domain and a coiled-coil dimerization module. Berger, P., Schaffitzel, C., Berger, I., Ban, N. & Suter, U. Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12177-82. Epub 2003 Oct 6. Europe PMC

Direct interaction of Ca2+/calmodulin inhibits histone deacetylase 5 repressor core binding to myocyte enhancer factor 2. Berger, I., Bieniossek, C., Schaffitzel, C., Hassler, M., Santelli, E. & Richmond, T.J. J Biol Chem. 2003 May 16;278(20):17625-35. Epub 2003 Mar 6. Europe PMC

In vitro generated antibodies specific for telomeric guanine-quadruplex DNA react with Stylonychia lemnae macronuclei. Schaffitzel, C., Berger, I., Postberg, J., Hanes, J., Lipps, H.J. & Pluckthun, A. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8572-7. Epub 2001 Jul 3. Europe PMC

Structural dynamics and cation interactions of DNA quadruplex molecules containing mixed guanine/cytosine quartets revealed by large-scale MD simulations. Spackova, N., Berger, I. & Sponer, J. J Am Chem Soc. 2001 Apr 11;123(14):3295-307. Europe PMC

Inter-strand C-H...O hydrogen bonds stabilizing four-stranded intercalated molecules: stereoelectronic effects of O4' in cytosine-rich DNA. Berger, I., Egli, M. & Rich, A. Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12116-21. Europe PMC

Crystal structure of the T4 regA translational regulator protein at 1.9 A resolution. Kang, C., Chan, R., Berger, I., Lockshin, C., Green, L., Gold, L. & Rich, A. Science. 1995 May 26;268(5214):1170-3. Europe PMC