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Staff at EMBL

Dr. Stephen Cusack

Head of Outstation and Senior Scientist
Cusack Group

EMBL Grenoble, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France

E-mail: send a mail


PhD 1976, Imperial College, London, UK.
Postdoctoral work at EMBL Grenoble.
Staff scientist at EMBL Grenoble 1980-1989.
Group Leader, Senior Scientist and Head of Outstation since 1989.
Director of EMBL-Grenoble University-CNRS Unit for Virus Host Cell Interactions (UVHCI) since 2007.
ERC Advanced Investigator.


RIG-I Self-Oligomerization Is Either Dispensable or Very Transient for Signal Transduction. Louber, J., Kowalinski, E., Bloyet, L.M., Brunel, J., Cusack, S. & Gerlier, D. PLoS One. 2014 Sep 26;9(9):e108770. doi: 10.1371/journal.pone.0108770.eCollection 2014. Europe PMC

RNA clamping by Vasa assembles a piRNA amplifier complex on transposon transcripts. Xiol, J., Spinelli, P., Laussmann, M.A., Homolka, D., Yang, Z., Cora, E., Coute, Y., Conn, S., Kadlec, J., Sachidanandam, R., Kaksonen, M., Cusack, S., Ephrussi, A. & Pillai, R.S. Cell. 2014 Jun 19;157(7):1698-711. doi: 10.1016/j.cell.2014.05.018. Epub 2014 Jun5. Europe PMC

The physiological target for LeuRS translational quality control is norvaline. Cvetesic, N., Palencia, A., Halasz, I., Cusack, S. & Gruic-Sovulj, I. EMBO J. 2014 Jun 16. pii: e201488199. Europe PMC

Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Dias, J., Van Nguyen, N., Georgiev, P., Gaub, A., Brettschneider, J., Cusack, S., Kadlec, J. & Akhtar, A. Genes Dev. 2014 May 1;28(9):929-42. doi: 10.1101/gad.240200.114. Europe PMC

Crystal structure of vaccinia virus mRNA capping enzyme provides insights into the mechanism and evolution of the capping apparatus. Kyrieleis, O.J., Chang, J., de la Pena, M., Shuman, S. & Cusack, S. Structure. 2014 Mar 4;22(3):452-65. doi: 10.1016/j.str.2013.12.014. Europe PMC

Structural and functional analysis of the three MIF4G domains of nonsense-mediated decay factor UPF2. Clerici, M., Deniaud, A., Boehm, V., Gehring, N.H., Schaffitzel, C. & Cusack, S. Nucleic Acids Res. 2014 Feb;42(4):2673-86. doi: 10.1093/nar/gkt1197. Epub 2013Nov 23. Europe PMC

Segmented negative strand RNA virus nucleoprotein structure. Reguera, J., Cusack, S. & Kolakofsky, D. Curr Opin Virol. 2014 Jan 29;5C:7-15. doi: 10.1016/j.coviro.2014.01.003. Europe PMC

Comparative structural and functional analysis of orthomyxovirus polymerase cap-snatching domains. Guilligay, D., Kadlec, J., Crepin, T., Lunardi, T., Bouvier, D., Kochs, G., Ruigrok, R.W. & Cusack, S. PLoS One. 2014 Jan 15;9(1):e84973. doi: 10.1371/journal.pone.0084973. eCollection2014 Jan 15. Europe PMC

CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Hallais, M., Pontvianne, F., Andersen, P.R., Clerici, M., Lener, D., Benbahouche Nel, H., Gostan, T., Vandermoere, F., Robert, M.C., Cusack, S., Verheggen, C., Jensen, T.H. & Bertrand, E. Nat Struct Mol Biol. 2013 Dec;20(12):1358-66. doi: 10.1038/nsmb.2720. Epub 2013Nov 24. Europe PMC

New 7-Methylguanine Derivatives Targeting the Influenza Polymerase PB2 Cap-Binding Domain. Pautus, S., Sehr, P., Lewis, J., Fortune, A., Wolkerstorfer, A., Szolar, O., Guilligay, D., Lunardi, T., Decout, J.L. & Cusack, S. J Med Chem. 2013 Nov 14;56(21):8915-30. doi: 10.1021/jm401369y. Epub 2013 Nov 6. Europe PMC

Molecular Basis for the Regulation of the H3K4 Methyltransferase Activity of PRDM9. Wu, H., Mathioudakis, N., Diagouraga, B., Dong, A., Dombrovski, L., Baudat, F., Cusack, S., de Massy, B. & Kadlec, J. Cell Rep. 2013 Oct 17;5(1):13-20. doi: 10.1016/j.celrep.2013.08.035. Epub 2013Oct 3. Europe PMC

Characterization of PA-N terminal domain of Influenza A polymerase reveals sequence specific RNA cleavage. Datta, K., Wolkerstorfer, A., Szolar, O.H., Cusack, S. & Klumpp, K. Nucleic Acids Res. 2013 Sep;41(17):8289-99. doi: 10.1093/nar/gkt603. Epub 2013Jul 11. Europe PMC

Systems to establish bunyavirus genome replication in the absence of transcription. Klemm, C., Reguera, J., Cusack, S., Zielecki, F., Kochs, G. & Weber, F. J Virol. 2013 Jul;87(14):8205-12. doi: 10.1128/JVI.00371-13. Epub 2013 May 22. Europe PMC

Structural basis for encapsidation of genomic RNA by La Crosse Orthobunyavirus nucleoprotein. Reguera, J., Malet, H., Weber, F. & Cusack, S. Proc Natl Acad Sci U S A. 2013 Apr 30;110(18):7246-51. doi:10.1073/pnas.1302298110. Epub 2013 Apr 15. Europe PMC

Leucyl-tRNA synthetase editing domain functions as a molecular rheostat to control codon ambiguity in Mycoplasma pathogens. Li, L., Palencia, A., Lukk, T., Li, Z., Luthey-Schulten, Z.A., Cusack, S., Martinis, S.A. & Boniecki, M.T. Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):3817-22. doi:10.1073/pnas.1218374110. Epub 2013 Feb 19. Europe PMC

Discovery of a Novel Class of Boron-Based Antibacterials with Activity against Gram-Negative Bacteria. Hernandez, V., Crepin, T., Palencia, A., Cusack, S., Akama, T., Baker, S.J., Bu, W., Feng, L., Freund, Y.R., Liu, L., Meewan, M., Mohan, M., Mao, W., Rock, F.L., Sexton, H., Sheoran, A., Zhang, Y., Zhang, Y.K., Zhou, Y., Nieman, J.A., Anugula, M.R., Keramane el, M., Savariraj, K., Reddy, D.S., Sharma, R., Subedi, R., Singh, R., O'Leary, A., Simon, N.L., De Marsh, P.L., Mushtaq, S., Warner, M., Livermore, D.M., Alley, M.R. & Plattner, J.J. Antimicrob Agents Chemother. 2013 Mar;57(3):1394-403. doi: 10.1128/AAC.02058-12.Epub 2013 Jan 7. Europe PMC

Plant tumour biocontrol agent employs a tRNA-dependent mechanism to inhibit leucyl-tRNA synthetase. Chopra, S., Palencia, A., Virus, C., Tripathy, A., Temple, B.R., Velazquez-Campoy, A., Cusack, S. & Reader, J.S. Nat Commun. 2013;4:1417. doi: 10.1038/ncomms2421. Europe PMC

Influenza A virus progeny vRNP trafficking in live infected cells studied with the virus-encoded fluorescently tagged PB2 protein. Avilov, S.V., Moisy, D., Naffakh, N. & Cusack, S. Vaccine. 2012 Dec 7;30(51):7411-7. doi: 10.1016/j.vaccine.2012.09.077. Epub 2012Oct 10. Europe PMC

A structure-based model of RIG-I activation. Kolakofsky, D., Kowalinski, E. & Cusack, S. RNA. 2012 Dec;18(12):2118-27. doi: 10.1261/rna.035949.112. Epub 2012 Nov 1. Europe PMC

Msl1-mediated dimerization of the dosage compensation complex is essential for male x-chromosome regulation in Drosophila. Hallacli, E., Lipp, M., Georgiev, P., Spielman, C., Cusack, S., Akhtar, A. & Kadlec, J. Mol Cell. 2012 Nov 30;48(4):587-600. doi: 10.1016/j.molcel.2012.09.014. Epub 2012Oct 18. Europe PMC

The multiple Tudor domain-containing protein TDRD1 is a molecular scaffold for mouse Piwi proteins and piRNA biogenesis factors. Mathioudakis, N., Palencia, A., Kadlec, J., Round, A., Tripsianes, K., Sattler, M., Pillai, R.S. & Cusack, S. RNA. 2012 Nov;18(11):2056-72. doi: 10.1261/rna.034181.112. Epub 2012 Sep 20. Europe PMC

Structural analysis of specific metal chelating inhibitor binding to the endonuclease domain of influenza pH1N1 (2009) polymerase. Kowalinski, E., Zubieta, C., Wolkerstorfer, A., Szolar, O.H., Ruigrok, R.W. & Cusack, S. PLoS Pathog. 2012;8(8):e1002831. doi: 10.1371/journal.ppat.1002831. Epub 2012 Aug2. Europe PMC

Structural dynamics of the aminoacylation and proofreading functional cycle of bacterial leucyl-tRNA synthetase. Palencia, A., Crepin, T., Vu, M.T., Lincecum TL, J.r, Martinis, S.A. & Cusack, S. Nat Struct Mol Biol. 2012 Jun 10;19(7):677-84. doi: 10.1038/nsmb.2317. Europe PMC

[RIG-I: a viral RNA detector molecular switch]. Kowalinski, E., Louber, J., Gerlier, D. & Cusack, S. Med Sci (Paris). 2012 Feb;28(2):136-8. doi: 10.1051/medsci/2012282006. Epub 2012Feb 27. Europe PMC

The structural basis for the integrity of adenovirus ad3 dodecahedron. Szolajska, E., Burmeister, W.P., Zochowska, M., Nerlo, B., Andreev, I., Schoehn, G., Andrieu, J.P., Fender, P., Naskalska, A., Zubieta, C., Cusack, S. & Chroboczek, J. PLoS One. 2012;7(9):e46075. doi: 10.1371/journal.pone.0046075. Epub 2012 Sep 25. Europe PMC

Replication competent influenza A virus encoding split-GFP tagged PB2 polymerase subunit allows live cell imaging of the viral life cycle. Avilov, S.V., Moisy, D., Munier, S., Schraidt, O., Naffakh, N. & Cusack, S. J Virol. 2012 Feb;86(3):1433-48. doi: 10.1128/JVI.05820-11. Epub 2011 Nov 23. Europe PMC

Structural basis for the activation of innate immune pattern-recognition receptor RIG-I by viral RNA. Kowalinski, E., Lunardi, T., McCarthy, A.A., Louber, J., Brunel, J., Grigorov, B., Gerlier, D. & Cusack, S. Cell. 2011 Oct 14;147(2):423-35. Europe PMC

Inducing phase changes in crystals of macromolecules: status and perspectives for controlled crystal dehydration. Russi, S., Juers, D.H., Sanchez-Weatherby, J., Pellegrini, E., Mossou, E., Forsyth, V.T., Huet, J., Gobbo, A., Felisaz, F., Moya, R., McSweeney, S.M., Cusack, S., Cipriani, F. & Bowler, M.W. J Struct Biol. 2011 Aug;175(2):236-43. Epub 2011 Mar 6. Europe PMC

Structural basis for MOF and MSL3 recruitment into the dosage compensation complex by MSL1. Kadlec, J., Hallacli, E., Lipp, M., Holz, H., Sanchez-Weatherby, J., Cusack, S. & Akhtar, A. Nat Struct Mol Biol. 2011 Feb;18(2):142-9. doi: 10.1038/nsmb.1960. Epub 2011 Jan9. Europe PMC

A Hybrid Structural Model of the Complete Brugia malayi Cytoplasmic Asparaginyl-tRNA Synthetase. Crepin, T., Peterson, F., Haertlein, M., Jensen, D., Wang, C., Cusack, S. & Kron, M. J Mol Biol. 2011 Jan 28;405(4):1056-69. Epub 2010 Dec 4. Europe PMC

Influenza A virus polymerase: structural insights into replication and host adaptation mechanisms. Boivin, S., Cusack, S., Ruigrok, R.W. & Hart, D.J. J Biol Chem. 2010 Sep 10;285(37):28411-7. doi: 10.1074/jbc.R110.117531. Epub 2010Jun 10. Europe PMC

Structure and RNA recognition by the snRNA and snoRNA transport factor PHAX. Mourao, A., Varrot, A., Mackereth, C.D., Cusack, S. & Sattler, M. RNA. 2010 Jun;16(6):1205-16. Epub 2010 Apr 29. Europe PMC

Towards an atomic resolution understanding of the influenza virus replication machinery. Ruigrok, R.W., Crepin, T., Hart, D.J. & Cusack, S. Curr Opin Struct Biol. 2010 Feb;20(1):104-13. Epub 2010 Jan 12. Europe PMC

Nuclear import and assembly of influenza A virus RNA polymerase studied in live cells by fluorescence cross-correlation spectroscopy. Huet, S., Avilov, S.V., Ferbitz, L., Daigle, N., Cusack, S. & Ellenberg, J. J Virol. 2010 Feb;84(3):1254-64. Epub 2009 Nov 11. Europe PMC

Bunyaviridae RNA polymerases (L-protein) have an N-terminal, influenza-like endonuclease domain, essential for viral cap-dependent transcription. Reguera, J., Weber, F. & Cusack, S. PLoS Pathog. 2010 Sep 16;6(9). pii: e1001101. Europe PMC

Avancées dans l'étude structurale et la compréhension moléculaire du mécanisme de transcription du virus de la grippe. Chevanas, S., Guilligay, D., Cusack, S., Hart, D.J., Ruigrok, R.W.H. & Crépin, T. Virologie 2010 14(6) 435-445

Crystal structures of the human and fungal cytosolic Leucyl-tRNA synthetase editing domains: A structural basis for the rational design of antifungal benzoxaboroles. Seiradake, E., Mao, W., Hernandez, V., Baker, S.J., Plattner, J.J., Alley, M.R. & Cusack, S. J Mol Biol. 2009 Jul 10;390(2):196-207. Epub 2009 May 6. Europe PMC

Structure and functional relevance of the Slit2 homodimerization domain. Seiradake, E., von Philipsborn, A.C., Henry, M., Fritz, M., Lortat-Jacob, H., Jamin, M., Hemrika, W., Bastmeyer, M., Cusack, S. & McCarthy, A.A. EMBO Rep. 2009 Jul;10(7):736-41. Epub 2009 Jun 5. Europe PMC

Design, synthesis, and evaluation of N-acyl modified sialic acids as inhibitors of adenoviruses causing epidemic keratoconjunctivitis. Johansson, S., Nilsson, E., Qian, W., Guilligay, D., Crepin, T., Cusack, S., Arnberg, N. & Elofsson, M. J Med Chem. 2009 Jun 25;52(12):3666-78. Europe PMC

Unusual bipartite mode of interaction between the nonsense-mediated decay factors, UPF1 and UPF2. Clerici, M., Mourao, A., Gutsche, I., Gehring, N.H., Hentze, M.W., Kulozik, A., Kadlec, J., Sattler, M. & Cusack, S. EMBO J. 2009 Jun 25;28(15):2293-2306. Europe PMC

Structure of SRP14 from the Schizosaccharomyces pombe signal recognition particle. Brooks, M.A., Ravelli, R.B., McCarthy, A.A., Strub, K. & Cusack, S. Acta Crystallogr D Biol Crystallogr. 2009 May;65(Pt 5):421-33. Epub 2009Apr 18. Europe PMC

The cap-snatching endonuclease of influenza virus polymerase resides in the PA subunit. Dias, A., Bouvier, D., Crepin, T., McCarthy, A.A., Hart, D.J., Baudin, F., Cusack, S. & Ruigrok, R.W. Nature. 2009 Apr 16;458(7240):914-8. Epub 2009 Feb 4. Europe PMC

[New strategy for targeting influenza virus replication] Dias, A., Bouvier, D., Cusack, S., Ruigrok, R.W. & Crepin, T. Med Sci (Paris). 2009 Apr;25(4):352-4. Europe PMC

Diverse role of three tyrosines in binding of the RNA 5' cap to the human nuclear cap binding complex. Worch, R., Jankowska-Anyszka, M., Niedzwiecka, A., Stepinski, J., Mazza, C., Darzynkiewicz, E., Cusack, S. & Stolarski, R. J Mol Biol. 2009 Jan 16;385(2):618-27. doi: 10.1016/j.jmb.2008.10.092. Epub 2008Nov 8. Europe PMC

The cell adhesion molecule "CAR" and sialic acid on human erythrocytes influence adenovirus in vivo biodistribution. Seiradake, E., Henaff, D., Wodrich, H., Billet, O., Perreau, M., Hippert, C., Mennechet, F., Schoehn, G., Lortat-Jacob, H., Dreja, H., Ibanes, S., Kalatzis, V., Wang, J.P., Finberg, R.W., Cusack, S. & Kremer, E.J. PLoS Pathog. 2009 Jan;5(1):e1000277. Epub 2009 Jan 2. Europe PMC

Structure of the C-terminal head domain of the fowl adenovirus type 1 short fibre. El Bakkouri, M., Seiradake, E., Cusack, S., Ruigrok, R.W. & Schoehn, G. Virology. 2008 Aug 15;378(1):169-76. Epub 2008 Jun 17. Europe PMC

Host determinant residue lysine 627 lies on the surface of a discrete, folded domain of influenza virus polymerase PB2 subunit. Tarendeau, F., Crepin, T., Guilligay, D., Ruigrok, R.W., Cusack, S. & Hart, D.J. PLoS Pathog. 2008 Aug 29;4(8):e1000136. Europe PMC

The structural basis for cap binding by influenza virus polymerase subunit PB2. Guilligay, D., Tarendeau, F., Resa-Infante, P., Coloma, R., Crepin, T., Sehr, P., Lewis, J.D., Ruigrok, R.W., Ortin, J., Hart, D.J. & Cusack, S. Nat Struct Mol Biol. 2008 May;15(5):500-6. Epub 2008 May 4. Europe PMC

[Trap the product to specifically inhibit the enzyme.] Crepin, T. & Cusack, S. Med Sci (Paris). 2007 Dec;23(12):1087-1089. Europe PMC

Structural insights into the mechanism and evolution of the vaccinia virus mRNA cap N7 methyl-transferase. De la Pena, M., Kyrieleis, O.J. & Cusack, S. EMBO J. 2007 Nov 28;26(23):4913-25. Epub 2007 Nov 8. Europe PMC

Structural insights into the Slit-Robo complex. Morlot, C., Thielens, N.M., Ravelli, R.B., Hemrika, W., Romijn, R.A., Gros, P., Cusack, S. & McCarthy, A.A. Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):14923-8. Epub 2007 Sep 11. Europe PMC

Production of Slit2 LRR domains in mammalian cells for structural studies and the structure of human Slit2 domain 3. Morlot, C., Hemrika, W., Romijn, R.A., Gros, P., Cusack, S. & McCarthy, A.A. Acta Crystallogr D Biol Crystallogr. 2007 Sep;63(Pt 9):961-8. Epub 2007Aug 17. Europe PMC

Cloning, expression, crystallization and preliminary X-ray analysis of the first two Ig domains from human roundabout 1 (Robo1). Morlot, C., Hemrika, W., Romijn, R.A., Gros, P., Cusack, S. & McCarthy, A.A. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Aug 1;63(Pt8):689-91. Epub 2007 Jul 21. Europe PMC

An antifungal agent inhibits an aminoacyl-tRNA synthetase by trapping tRNA in the editing site. Rock, F.L., Mao, W., Yaremchuk, A., Tukalo, M., Crepin, T., Zhou, H., Zhang, Y.K., Hernandez, V., Akama, T., Baker, S.J., Plattner, J.J., Shapiro, L., Martinis, S.A., Benkovic, S.J., Cusack, S. & Alley, M.R. Science. 2007 Jun 22;316(5832):1759-61. Europe PMC

Three-dimensional model for the isolated recombinant influenza virus polymerase heterotrimer. Torreira, E., Schoehn, G., Fernandez, Y., Jorba, N., Ruigrok, R.W., Cusack, S., Ortin, J. & Llorca, O. Nucleic Acids Res. 2007;35(11):3774-83. Epub 2007 May 21. Europe PMC

Structure of dimeric SecA, the Escherichia coli preprotein translocase motor. Papanikolau, Y., Papadovasilaki, M., Ravelli, R.B., McCarthy, A.A., Cusack, S., Economou, A. & Petratos, K. J Mol Biol. 2007 Mar 9;366(5):1545-57. Epub 2006 Dec 23. Europe PMC

Structure and nuclear import function of the C-terminal domain of influenza virus polymerase PB2 subunit. Tarendeau, F., Boudet, J., Guilligay, D., Mas, P.J., Bougault, C.M., Boulo, S., Baudin, F., Ruigrok, R.W., Daigle, N., Ellenberg, J., Cusack, S., Simorre, J.P. & Hart, D.J. Nat Struct Mol Biol. 2007 Mar;14(3):229-33. Epub 2007 Feb 25. Europe PMC

A methodology and an instrument for the temperature-controlled optimization of crystal growth. Budayova-Spano, M., Dauvergne, F., Audiffren, M., Bactivelane, T. & Cusack, S. Acta Crystallogr D Biol Crystallogr. 2007 Mar;63(Pt 3):339-47. Epub 2007Feb 21. Europe PMC

Solution structure of NOD1 CARD and mutational analysis of its interaction with the CARD of downstream kinase RICK. Manon, F., Favier, A., Nunez, G., Simorre, J.P. & Cusack, S. J Mol Biol. 2007 Jan 5;365(1):160-74. Epub 2006 Sep 29. Europe PMC

Structural and mutational analysis of human Ad37 and canine adenovirus 2 fiber heads in complex with the D1 domain of coxsackie and adenovirus receptor. Seiradake, E., Lortat-Jacob, H., Billet, O., Kremer, E.J. & Cusack, S. J Biol Chem. 2006 Nov 3;281(44):33704-16. Epub 2006 Aug 21. Europe PMC

High-throughput sample handling and data collection at synchrotrons: embedding the ESRF into the high-throughput gene-to-structure pipeline. Beteva, A., Cipriani, F., Cusack, S., Delageniere, S., Gabadinho, J., Gordon, E.J., Guijarro, M., Hall, D.R., Larsen, S., Launer, L., Lavault, C.B., Leonard, G.A., Mairs, T., McCarthy, A., McCarthy, J., Meyer, J., Mitchell, E., Monaco, S., Nurizzo, D., Pernot, P., Pieritz, R., Ravelli, R.G., Rey, V., Shepard, W., Spruce, D., Stuart, D.I., Svensson, O., Theveneau, P., Thibault, X., Turkenburg, J., Walsh, M. & McSweeney, S.M. Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1162-9. Epub 2006 Sep 19. Europe PMC

Structural genomics of the Epstein-Barr virus. Tarbouriech, N., Buisson, M., Geoui, T., Daenke, S., Cusack, S. & Burmeister, W.P. Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1276-85. Epub 2006Sep 19. Europe PMC

Crystal structure of the UPF2-interacting domain of nonsense-mediated mRNA decay factor UPF1. Kadlec, J., Guilligay, D., Ravelli, R.B. & Cusack, S. RNA. 2006 Oct;12(10):1817-24. Epub 2006 Aug 24. Europe PMC

Structures of two bacterial prolyl-tRNA synthetases with and without a cis-editing domain. Crepin, T., Yaremchuk, A., Tukalo, M. & Cusack, S. Structure. 2006 Oct;14(10):1511-25. Europe PMC

Automation of sample mounting for macromolecular crystallography. Cipriani, F., Felisaz, F., Launer, L., Aksoy, J.S., Caserotto, H., Cusack, S., Dallery, M., di-Chiaro, F., Guijarro, M., Huet, J., Larsen, S., Lentini, M., McCarthy, J., McSweeney, S., Ravelli, R., Renier, M., Taffut, C., Thompson, A., Leonard, G.A. & Walsh, M.A. Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1251-9. Epub 2006Sep 19. Europe PMC

First steps towards effective methods in exploiting high-throughput technologies for the determination of human protein structures of high biomedical value. Banci, L., Bertini, I., Cusack, S., de Jong, R.N., Heinemann, U., Jones, E.Y., Kozielski, F., Maskos, K., Messerschmidt, A., Owens, R., Perrakis, A., Poterszman, A., Schneider, G., Siebold, C., Silman, I., Sixma, T., Stewart-Jones, G., Sussman, J.L., Thierry, J.C. & Moras, D. Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1208-17. Epub 2006Sep 19. Europe PMC

Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera. Zubieta, C., Blanchoin, L. & Cusack, S. FEBS J. 2006 Sep;273(18):4336-45. Europe PMC

Discovering new classes of Brugia malayi Asparaginyl-tRNA synthetase inhibitors and relating specificity to conformational change. Sukuru, S.C., Crepin, T., Milev, Y., Marsh, L.C., Hill, J.B., Anderson, R.J., Morris, J.C., Rohatgi, A., O'mahony, G., Grotli, M., Danel, F., Page, M.G., Hartlein, M., Cusack, S., Kron, M.A. & Kuhn, L.A. J Comput Aided Mol Des. 2006 Mar;20(3):159-78. Epub 2006 Apr 28. Europe PMC

Inhibition of mRNA Deadenylation by the Nuclear Cap Binding Complex (CBC). Balatsos, N.A., Nilsson, P., Mazza, C., Cusack, S. & Virtanen, A. J Biol Chem. 2006 Feb 17;281(7):4517-22. Epub 2005 Nov 28. Europe PMC

Contributions of conformational flexibility to Brugia malayi asparaginyl-tRNA synthetase specificity for inhibitors identified by structure-based screening. Sukuru, SCK; Crepin, T; Morris, JC; Grotli, M; Danel, F; Page, MGP; Haertlein, M; Cusack, S.; Kron, MA; Kuhn, LA. Abstracts of papers of the American Chemical Society 231 March 2006 (231)

Crystal structure of enteric adenovirus serotype 41 short fiber head. Seiradake, E. & Cusack, S. J Virol 2005 Nov;79(22):14088-94. Europe PMC

The crystal structure of leucyl-tRNA synthetase complexed with tRNA(Leu) in the post-transfer-editing conformation. Tukalo, M., Yaremchuk, A., Fukunaga, R., Yokoyama, S. & Cusack, S. Nat Struct Mol Biol 2005 Oct;12(10):923-930. Epub 2005 Sep 11. Europe PMC

Specificity of recognition of mRNA 5' cap by human nuclear cap-binding complex. Worch, R., Niedzwiecka, A., Stepinski, J., Mazza, C., Jankowska-Anyszka, M., Darzynkiewicz, E., Cusack, S. & Stolarski, R. RNA 2005 Sep;11(9):1355-63. Epub 2005 Jul 25. Europe PMC

The Monomeric dUTPase from Epstein-Barr Virus Mimics Trimeric dUTPases. Tarbouriech, N., Buisson, M., Seigneurin, J.M., Cusack, S. & Burmeister, W.P. Structure (Camb) 2005 Sep;13(9):1299-310. Europe PMC

X-ray crystallographic and NMR studies of the third KH domain of hnRNP K in complex with single-stranded nucleic acids. Backe, P.H., Messias, A.C., Ravelli, R.B.G., Sattler, M. & Cusack, S. Structure (Camb) 2005 Jul;13(7):1055-67. Europe PMC

Significance of the first transcribed nucleoside of capped RNA for ligand-induced folding of the cap-binding complex. Worch, R., Niedzwiecka, A., Stepinski, J., Jankowska-Anyszka, M., Mazza, C., Darzynkiewicz, E., Cusack, S. & Stolarski, R. Journal of Physics-condensed Matter 2005 May;17(18):1495-1502.

A quasi-atomic model of human adenovirus type 5 capsid. Fabry, C.M., Rosa-Calatrava, M., Conway, J.F., Zubieta, C., Cusack, S., Ruigrok, R.W. & Schoehn, G. EMBO J 2005 May 4;24(9):1645-54. Epub 2005 Apr 21. Europe PMC

Prolyl-tRNA synthetase. Musier-Forsyth, K., Burke, B. & Cusack, S. In: "Aminoacyl-tRNA Synthetases", Ibba, M., Francklyn, C., & Cusack, S. (Eds.), Landes Bioscience, 2005

Adenovirus complex structures. Cusack, S. Curr Opin Struct Biol 2005 Apr;15(2):237-43. Europe PMC

The structure of the human adenovirus 2 penton. Zubieta, C., Schoehn, G., Chroboczek, J. & Cusack, S. Mol Cell 2005 Jan 7;17(1):121-35. Europe PMC

Novel way of capping mRNA trimer and studies of its interaction with human nuclear cap-binding complex. Worch, R., Stepinski, J., Niedzwiecka, A., Jankowska-Anyszka, M., Mazza, C., Cusack, S., Stolarski, R. & Darzynkiewicz, E. Nucleosides Nucleotides Nucleic Acids 2005;24(5-7):1131-4. Europe PMC

Crystal structure of species D adenovirus fiber knobs and their sialic acid binding sites. Burmeister, W.P., Guilligay, D., Cusack, S., Wadell, G. & Arnberg, N. J Virol 2004 Jul;78(14):7727-36. Europe PMC

The structural basis for the interaction between nonsense-mediated mRNA decay factors UPF2 and UPF3. Kadlec, J., Izaurralde, E. & Cusack, S. Nat Struct Mol Biol 2004 Apr;11(4):330-7. Epub 2004 Mar 7. Europe PMC

Crystallization, microPIXE and preliminary crystallographic analysis of the complex between the third KH domain of hnRNP K and single-stranded DNA. Backe, P.H., Ravelli, R.B.G., Garman, E. & Cusack, S. Acta Crystallogr D Biol Crystallogr 2004 Apr;60(Pt 4):784-7. Epub 2004 Mar23. Europe PMC

Seryl-tRNA synthetase. Weygand Durasevic, I. & Cusack, S. In: "Aminoacyl-tRNA Synthetasees", Ibba, M., Francklyn, C., & Cusack, S. (Eds.), Landes Biosciences, 2004

Conserved tertiary base pairing ensures proper RNA folding and efficient assembly of the signal recognition particle Alu domain. Huck, L., Scherrer, A., Terzi, L., Johnson, A.E., Bernstein, H.D., Cusack, S., Weichenrieder, O. & Strub, K. Nucleic Acids Res 2004 Sep 21;32(16):4915-24. Print 2004. Europe PMC

Structural basis for orthogonal tRNA specificities of tyrosyl-tRNA synthetases for genetic code expansion. Kobayashi, T., Nureki, O., Ishitani, R., Yaremchuk, A., Tukalo, M., Cusack, S., Sakamoto, K. & Yokoyama, S. Nat Struct Biol 2003 Jun;10(6):425-32. Europe PMC

Structural and mechanistic basis of pre- and posttransfer editing by leucyl-tRNA synthetase. Lincecum, T.L., Tukalo, M., Yaremchuk, A., Mursinna, R.S., Williams, A.M., Sproat, B.S., Van Den Eynde, W., Link, A., Van Calenbergh, S., Grotli, M., Martinis, S.A. & Cusack, S. Mol Cell 2003 Apr;11(4):951-63. Europe PMC

Structural analysis of the adaptor protein ClpS in complex with the N-terminal domain of ClpA. Zeth, K., Ravelli, R.B., Paal, K., Cusack, S., Bukau, B. & Dougan, D.A. Nat Struct Biol 2002 Dec;9(12):906-11. Europe PMC

Co-crystallization of the human nuclear cap-binding complex with a m7GpppG cap analogue using protein engineering. Mazza, C., Segref, A., Mattaj, I.W. & Cusack, S. Acta Crystallogr D Biol Crystallogr 2002 Dec;58(Pt 12):2194-7. Europe PMC

Large-scale induced fit recognition of an m(7)GpppG cap analogue by the human nuclear cap-binding complex. Mazza, C., Segref, A., Mattaj, I.W. & Cusack, S. EMBO J 2002 Oct 15;21(20):5548-57. Europe PMC

Class I tyrosyl-tRNA synthetase has a class II mode of cognate tRNA recognition. Yaremchuk, A., Kriklivyi, I., Tukalo, M. & Cusack, S. EMBO J 2002 Jul 15;21(14):3829-40. Europe PMC

Crystallization and preliminary X-ray analysis of the Escherichia coli adaptor protein ClpS, free and in complex with the N-terminal domain of ClpA. Zeth, K., Dougan, D.A., Cusack, S., Bukau, B. & Ravelli, R.B. Acta Crystallogr D Biol Crystallogr 2002 Jul;58(Pt 7):1207-10. Europe PMC

tRNA-like recognition of group I introns by a tyrosyl-tRNA synthetase. Myers, C.A., Kuhla, B., Cusack, S. & Lambowitz, A.M. Proc Natl Acad Sci U S A 2002 Mar 5;99(5):2630-5. Europe PMC

Towards the structure of the mammalian signal recognition particle. Wild, K., Weichenrieder, O., Strub, K., Sinning, I. & Cusack, S. Curr Opin Struct Biol 2002 Feb;12(1):72-81. Europe PMC

Crystal structure of an early protein-RNA assembly complex of the signal recognition particle. Wild, K., Sinning, I. & Cusack, S. Science 2001 Oct 19;294(5542):598-601. Europe PMC

Crystal structure of the human nuclear cap binding complex. Mazza, C., Ohno, M., Segref, A., Mattaj, I.W. & Cusack, S. Mol Cell 2001 Aug;8(2):383-96. Europe PMC

Structure of the fiber head of Ad3, a non-CAR-binding serotype of adenovirus. Durmort, C., Stehlin, C., Schoehn, G., Mitraki, A., Drouet, E., Cusack, S. & Burmeister, W.P. Virology 2001 Jul 5;285(2):302-12. Europe PMC

A succession of substrate induced conformational changes ensures the amino acid specificity of Thermus thermophilus prolyl-tRNA synthetase: comparison with histidyl-tRNA synthetase. Yaremchuk, A., Tukalo, M., Grotli, M. & Cusack, S. J Mol Biol 2001 Jun 15;309(4):989-1002. Europe PMC

Hierarchical assembly of the Alu domain of the mammalian signal recognition particle. Weichenrieder, O., Stehlin, C., Kapp, U., Birse, D.E., Timmins, P.A., Strub, K. & Cusack, S. RNA 2001 May;7(5):731-40. Europe PMC

Kinetic analysis of adenovirus fiber binding to its receptor reveals an avidity mechanism for trimeric receptor-ligand interactions. Lortat-Jacob, H., Chouin, E., Cusack, S. & van Raaij, M.J. J Biol Chem 2001 Mar 23;276(12):9009-15. Europe PMC

Influence of adenoviral fiber mutations on viral encapsidation, infectivity and in vivo tropism. Leissner, P., Legrand, V., Schlesinger, Y., Hadji, D.A., van Raaij, M., Cusack, S., Pavirani, A. & Mehtali, M. Gene Ther 2001 Jan;8(1):49-57. Europe PMC

Structure, folding and assembly of adenovirus fibers. Mitraki, A., van Raaij, M., Ruigrok, R., Cusack, S., Hernandez, J.-F. & Luckey, M. In "Self-assembling Peptide Systems in Biology, Medicine and Engineering," Kluwer Academic Publishers, Dordrecht, The Netherlands

Structure and assembly of the Alu domain of the mammalian signal recognition particle. Weichenrieder, O., Wild, K., Strub, K. & Cusack, S. Nature 2000 Nov 9;408(6809):167-73. Europe PMC

Dimeric structure of the coxsackievirus and adenovirus receptor D1 domain at 1.7 A resolution. van Raaij, M.J., Chouin, E., van der Zandt, H., Bergelson, J.M. & Cusack, S. Structure Fold Des 2000 Nov 15;8(11):1147-55. Europe PMC

Mutual conformational adaptations in antigen and antibody upon complex formation between an Fab and HIV-1 capsid protein p24. Monaco-Malbet, S., Berthet-Colominas, C., Novelli, A., Battai, N., Piga, N., Cheynet, V., Mallet, F. & Cusack, S. Structure Fold Des 2000 Oct 15;8(10):1069-77. Europe PMC

Crystal structure of a eukaryote/archaeon-like protyl-tRNA synthetase and its complex with tRNAPro(CGG) Yaremchuk, A., Cusack, S. & Tukalo, M. EMBO J 2000 Sep 1;19(17):4745-58. Europe PMC

The 2 A crystal structure of leucyl-tRNA synthetase and its complex with a leucyl-adenylate analogue. Cusack, S., Yaremchuk, A. & Tukalo, M. EMBO J 2000 May 15;19(10):2351-61 Europe PMC

Crystallization and preliminary crystallographic analysis of Thermus thermophilus prolyl-tRNA synthetase Yaremchuk, A., Cusack, S., Gudzera, O., Grotli, M. & Tukalo, M. Acta Crystallogr D Biol Crystallogr 2000 May;56 ( Pt 5):667-9 Europe PMC

Improved crystals of Thermus thermophilus prolyl-tRNA synthetase complexed with cognate tRNA obtained by crystallization from precipitate. Yaremchuk, A., Kriklivyi, I., Cusack, S. & Tukalo, M. Acta Crystallogr D Biol Crystallogr 2000 Feb;56 ( Pt 2):197-9 Europe PMC

Crystallization and preliminary X-ray diffraction analysis of Thermus thermophilus prolyl-tRNA synthetase. Yaremchuk, A., Cusack, S. & Tukalo, M. Acta Crystallogr D Biol Crystallogr 2000 Feb;56 ( Pt 2):195-6 Europe PMC

The 2 A structure of helix 6 of the human signal recognition particle Wild, K., Weichenrieder, O., Leonard, G.A. & Cusack, S. Structure Fold Des 1999 Nov 15;7(11):1345-52 Europe PMC

A triple beta-spiral in the adenovirus fibre shaft reveals a new structural motif for a fibrous protein. van Raaij, M.J., Mitraki, A., Lavigne, G. & Cusack, S. Nature 1999 Oct 28;401(6756):935-8 Europe PMC

Protein microcrystals and the design of a microdiffractometer: current experience and plans at EMBL and ESRF/ID13. Perrakis, A., Cipriani, F., Castagna, J.C., Claustre, L., Burghammer, M., Riekel, C. & Cusack, S. Acta Crystallogr D Biol Crystallogr 1999 Oct;55 ( Pt 10):1765-70 Europe PMC

Structure of the human adenovirus serotype 2 fiber head domain at 1.5 A resolution. van Raaij, M.J., Louis, N., Chroboczek, J. & Cusack, S. Virology 1999 Sep 30;262(2):333-43 Europe PMC

Head-to-tail dimers and interdomain flexibility revealed by the crystal structure of HIV-1 capsid protein (p24) complexed with a monoclonal antibody Fab. Berthet-Colominas, C., Monaco, S., Novelli, A., Sibai, G., Mallet, F. & Cusack, S. EMBO J 1999 Mar 1;18(5):1124-36 Europe PMC

RNA-protein complexes. Cusack, S. Curr Opin Struct Biol 1999 Feb;9(1):66-73 Europe PMC

Escherichia coli SecA shape and dimensions. Shilton, B., Svergun, D.I., Volkov, V.V., Koch, M.H., Cusack, S. & Economou, A. FEBS Lett 1998 Oct 2 436(2) 277-282 Europe PMC

Small is beautiful: protein micro-crystallography. Cusack, S., Belrhali, H., Bram, A., Burghammer, M., Perrakis, A. & Riekel, C. Nat Struct Biol 1998 Aug 5 Suppl 634-637 Europe PMC

The crystal structure of asparaginyl-tRNA synthetase from Thermus thermophilus and its complexes with ATP and asparaginyl-adenylate: the mechanism of discrimination between asparagine and aspartic acid. Berthet-Colominas, C., Seignovert, L., Hartlein, M., Grotli, M., Cusack, S. & Leberman, R. EMBO J 1998 May 15 17(10) 2947-2960 Europe PMC

tRNA(Pro) anticodon recognition by Thermus thermophilus prolyl-tRNA synthetase. Cusack, S., Yaremchuk, A., Krikliviy, I. & Tukalo, M. Structure 1998 Jan 15 6(1) 101-108 Europe PMC

tRNA recognition by aminoacyl-tRNA synthetases. Cusack, S., Yaremchuk, A. & Tukalo, M. IN: The many faces of tRNA. Eggleston, D.S., Prescott, C.D. & Pearson, N.D., eds. Academic Press, London, pp 55-65

The crystal structure of the signal recognition particle Alu RNA binding heterodimer, SRP9/14. Birse, D.E., Kapp, U., Strub, K., Cusack, S. & Aberg, A. EMBO J 1997 Jul 1 16(13) 3757-3766 Europe PMC

Crystal structure analysis of the activation of histidine by Thermus thermophilus histidyl-tRNA synthetase. Aberg, A., Yaremchuk, A., Tukalo, M., Rasmussen, B. & Cusack, S. Biochemistry 1997 Mar 18;36(11):3084-94 Europe PMC

Identification of a minimal Alu RNA folding domain that specifically binds SRP9/14. Weichenrieder, O., Kapp, U., Cusack, S. & Strub, K. RNA. 1997 Nov;3(11):1262-74. Europe PMC

The crystal structures of T. thermophilus lysyl-tRNA synthetase complexed with E. coli tRNA(Lys) and a T. thermophilus tRNA(Lys) transcript: anticodon recognition and conformational changes upon binding of a lysyl-adenylate analogue. Cusack, S., Yaremchuk, A. & Tukalo, M. EMBO J 1996 Nov 15;15(22):6321-34 Europe PMC

The crystal structure of the ternary complex of T.thermophilus seryl- tRNA synthetase with tRNA(Ser) and a seryl-adenylate analogue reveals a conformational switch in the active site. Cusack, S., Yaremchuk, A. & Tukalo, M. EMBO J 1996 Jun 3;15(11):2834-42 Europe PMC

Crystallization of Thermus thermophilus histidyl-tRNA synthetase and its complex with tRNAHis. Yaremchuk, A.D., Cusack, S., Aberg, A., Gudzera, O., Kryklivyi, I. & Tukalo, M. Proteins 1995 Aug;22(4):426-8 Europe PMC

The structural basis for seryl-adenylate and Ap4A synthesis by seryl- tRNA synthetase. Belrhali, H., Yaremchuk, A., Tukalo, M., Berthet-Colominas, C., Rasmussen, B., Bosecke, P., Diat, O. & Cusack, S. Structure 1995 Apr 15;3(4):341-52 Europe PMC

Cocrystallization of lysyl-tRNA synthetase from Thermus thermophilus with its cognate tRNAlys and with Escherichia coli tRNAlys. Yaremchuk, A.D., Krikliviy, I.A., Cusack, S. & Tukalo, M.A. Proteins 1995 Mar;21(3):261-4 Europe PMC

Structure of influenza virus RNP. I. Influenza virus nucleoprotein melts secondary structure in panhandle RNA and exposes the bases to the solvent. Baudin, F., Bach, C., Cusack, S. & Ruigrok, R.W. EMBO J 1994 Jul 1;13(13):3158-65 Europe PMC

The 2.9 A crystal structure of T. thermophilus seryl-tRNA synthetase complexed with tRNA(Ser). Biou, V., Yaremchuk, A., Tukalo, M. & Cusack, S. Science 1994 Mar 11 263(5152) 1404-1410 Europe PMC

A small percentage of influenza virus M1 protein contains zinc but zinc does not influence in vitro M1-RNA interaction. Elster, C., Fourest, E., Baudin, F., Larsen, K., Cusack, S. & Ruigrok, R.W. J Gen Virol 1994 Jan;75 ( Pt 1):37-42 Europe PMC

Comparison of structure and sequence of influenza B/Yamagata and B/Beijing neuraminidases shows a conserved "head" but much greater variability in the "stalk" and NB protein. Burmeister, W.P., Baudin, F., Cusack, S. & Ruigrok, R.W. Virology 1993 Feb;192(2):683-6 Europe PMC

The 2.2 A resolution crystal structure of influenza B neuraminidase and its complex with sialic acid. Burmeister, W.P., Ruigrok, R.W. & Cusack, S. EMBO J 1992 Jan;11(1):49-56 Europe PMC

A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 A Cusack, S., Berthet-Colominas, C., Hartlein, M., Nassar, N. & Leberman, R. Nature 1990 Sep 20 347(6290) 249-255 Europe PMC