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Staff at EMBL

Dr. Darren Hart

Visiting Team Leader
Hart Team (Visiting)

EMBL Grenoble, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France

E-mail: send a mail


PhD 1996, Oxford University.
Postdoctoral research at Cambridge University.
Group Leader at Sense Proteomic Ltd., Cambridge, UK.
Team Leader at EMBL Grenoble 2003-2014.
Visiting Team Leader since 2014.


Structural Basis for the Oligomerization of the MADS Domain Transcription Factor SEPALLATA3 in Arabidopsis. Puranik, S., Acajjaoui, S., Conn, S., Costa, L., Conn, V., Vial, A., Marcellin, R., Melzer, R., Brown, E., Hart, D., Theissen, G., Silva, C.S., Parcy, F., Dumas, R., Nanao, M. & Zubieta, C. Plant Cell. 2014 Sep 16. pii: tpc.114.127910. Europe PMC

Structural and Biophysical Characterization of Murine Rif1 C Terminus Reveals High Specificity for DNA Cruciform Structures. Sukackaite, R., Jensen, M.R., Mas, P.J., Blackledge, M., Buonomo, S.B. & Hart, D.J. J Biol Chem. 2014 Mar 14. Europe PMC

Library methods for structural biology of challenging proteins and their complexes. Hart, D.J. & Waldo, G.S. Curr Opin Struct Biol. 2013 Jun;23(3):403-8. doi: 10.1016/ Epub2013 Apr 17. Europe PMC

Development of a peptide that selectively activates protein phosphatase-1 in living cells. Chatterjee, J., Beullens, M., Sukackaite, R., Qian, J., Lesage, B., Hart, D.J., Bollen, M. & Köhn, M. Angew Chem Int Ed Engl. 2012 Oct 1;51(40):10054-9. doi: 10.1002/anie.201204308.Epub 2012 Sep 7. Europe PMC

Structure of the phosphatase domain of the cell fate determinant SpoIIE from Bacillus subtilis. Levdikov, V.M., Blagova, E.V., Rawlings, A.E., Jameson, K., Tunaley, J., Hart, D.J., Barak, I. & Wilkinson, A.J. J Mol Biol. 2012 Jan 13;415(2):343-58. doi: 10.1016/j.jmb.2011.11.017. Epub 2011Nov 15. Europe PMC

Nucleoporin Nup50 stabilizes closed conformation of armadillo repeat 10 in importin alpha5. Pumroy, R.A., Nardozzi, J.D., Hart, D.J., Root, M.J. & Cingolani, G. J Biol Chem. 2012 Jan 13;287(3):2022-31. Epub 2011 Nov 30. Europe PMC

Library-based methods for identification of soluble expression constructs. Yumerefendi, H., Desravines, D.C. & Hart, D.J. Methods. 2011 Sep;55(1):38-43. Epub 2011 Jun 23. Europe PMC

ORF-selector ESPRIT: a second generation library screen for soluble protein expression employing precise open reading frame selection. An, Y., Yumerefendi, H., Mas, P.J., Chesneau, A. & Hart, D.J. J Struct Biol. 2011 Aug;175(2):189-97. doi: 10.1016/j.jsb.2011.04.004. Epub 2011Apr 16. Europe PMC

Interaction of the influenza A virus polymerase PB2 C-terminal region with importin alpha isoforms provides insights into host adaptation and polymerase assembly. Boivin, S. & Hart, D.J. J Biol Chem. 2011 Mar 25;286(12):10439-48. doi: 10.1074/jbc.M110.182964. Epub2011 Jan 7. Europe PMC

CoESPRIT: a library-based construct screening method for identification and expression of soluble protein complexes. An, Y., Meresse, P., Mas, P.J. & Hart, D.J. PLoS One. 2011 Feb 22;6(2):e16261. doi: 10.1371/journal.pone.0016261. Europe PMC

Expression of soluble, active fragments of the morphogenetic protein SpoIIE from Bacillus subtilis using a library-based construct screen. Rawlings, A.E., Levdikov, V.M., Blagova, E., Colledge, V.L., Mas, P.J., Tunaley, J., Vavrova, L., Wilson, K.S., Barak, I., Hart, D.J. & Wilkinson, A.J. Protein Eng Des Sel. 2010 Nov;23(11):817-25. doi: 10.1093/protein/gzq057. Epub2010 Sep 3. Europe PMC

ESPRIT: an automated, library-based method for mapping and soluble expression of protein domains from challenging targets. Yumerefendi, H., Tarendeau, F., Mas, P.J. & Hart, D.J. J Struct Biol. 2010 Oct;172(1):66-74. doi: 10.1016/j.jsb.2010.02.021. Epub 2010Mar 4. Europe PMC

Structure and inhibition of herpesvirus DNA packaging terminase nuclease domain. Nadal, M., Mas, P.J., Blanco, A.G., Arnan, C., Sola, M., Hart, D.J. & Coll, M. Proc Natl Acad Sci U S A. 2010 Sep 14;107(37):16078-83. doi:10.1073/pnas.1007144107. Epub 2010 Aug 30. Europe PMC

Influenza A virus polymerase: structural insights into replication and host adaptation mechanisms. Boivin, S., Cusack, S., Ruigrok, R.W. & Hart, D.J. J Biol Chem. 2010 Sep 10;285(37):28411-7. doi: 10.1074/jbc.R110.117531. Epub 2010Jun 10. Europe PMC

Biological and biophysical properties of the histone deacetylase inhibitor suberoylanilide hydroxamic Acid are affected by the presence of short alkyl groups on the phenyl ring. Oger, F., Lecorgne, A., Sala, E., Nardese, V., Demay, F., Chevance, S., Desravines, D.C., Aleksandrova, N., Guevel, R.L., Lorenzi, S., Beccari, A.R., Barath, P., Hart, D.J., Bondon, A., Carettoni, D., Simonneaux, G. & Salbert, G. J Med Chem. 2010 Mar 11;53(5):1937-50. Europe PMC

Towards an atomic resolution understanding of the influenza virus replication machinery. Ruigrok, R.W., Crepin, T., Hart, D.J. & Cusack, S. Curr Opin Struct Biol. 2010 Feb;20(1):104-13. Epub 2010 Jan 12. Europe PMC

Avancées dans l'étude structurale et la compréhension moléculaire du mécanisme de transcription du virus de la grippe. Chevanas, S., Guilligay, D., Cusack, S., Hart, D.J., Ruigrok, R.W.H. & Crépin, T. Virologie 2010 14(6) 435-445

Cooperative binding of two acetylation marks on a histone tail by a single bromodomain. Moriniere, J., Rousseaux, S., Steuerwald, U., Soler-Lopez, M., Curtet, S., Vitte, A.L., Govin, J., Gaucher, J., Sadoul, K., Hart, D.J., Krijgsveld, J., Khochbin, S., Muller, C.W. & Petosa, C. Nature. 2009 Oct 1;461(7264):664-8. Europe PMC

Solubility survey of fragments of the neurofibromatosis type 1 protein neurofibromin. Bonneau, F., Lenherr, E.D., Pena, V., Hart, D.J. & Scheffzek, K. Protein Expr Purif. 2009 May;65(1):30-7. Epub 2008 Dec 10. Europe PMC

The cap-snatching endonuclease of influenza virus polymerase resides in the PA subunit. Dias, A., Bouvier, D., Crepin, T., McCarthy, A.A., Hart, D.J., Baudin, F., Cusack, S. & Ruigrok, R.W. Nature. 2009 Apr 16;458(7240):914-8. Epub 2009 Feb 4. Europe PMC

Expression of Helicobacter pylori CagA domains by library-based construct screening. Angelini, A., Tosi, T., Mas, P., Acajjaoui, S., Zanotti, G., Terradot, L. & Hart, D.J. FEBS J. 2009 Feb;276(3):816-24. Europe PMC

Host determinant residue lysine 627 lies on the surface of a discrete, folded domain of influenza virus polymerase PB2 subunit. Tarendeau, F., Crepin, T., Guilligay, D., Ruigrok, R.W., Cusack, S. & Hart, D.J. PLoS Pathog. 2008 Aug 29;4(8):e1000136. Europe PMC

The structural basis for cap binding by influenza virus polymerase subunit PB2. Guilligay, D., Tarendeau, F., Resa-Infante, P., Coloma, R., Crepin, T., Sehr, P., Lewis, J.D., Ruigrok, R.W., Ortin, J., Hart, D.J. & Cusack, S. Nat Struct Mol Biol. 2008 May;15(5):500-6. Epub 2008 May 4. Europe PMC

The impact of protein expression methodologies on structural proteomics. Chesneau, A., Yumerefendi, H. & Hart, D.J. in Structural Proteomics, Sussman, J.L. & Silman, I. (eds.), World Scientific Publishing Company, Singapore, 2008, pp. 572

Structure and nuclear import function of the C-terminal domain of influenza virus polymerase PB2 subunit. Tarendeau, F., Boudet, J., Guilligay, D., Mas, P.J., Bougault, C.M., Boulo, S., Baudin, F., Ruigrok, R.W., Daigle, N., Ellenberg, J., Cusack, S., Simorre, J.P. & Hart, D.J. Nat Struct Mol Biol. 2007 Mar;14(3):229-33. Epub 2007 Feb 25. Europe PMC

Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE. Alzari, P.M., Berglund, H., Berrow, N.S., Blagova, E., Busso, D., Cambillau, C., Campanacci, V., Christodoulou, E., Eiler, S., Fogg, M.J., Folkers, G., Geerlof, A., Hart, D., Haouz, A., Herman, M.D., Macieira, S., Nordlund, P., Perrakis, A., Quevillon-Cheruel, S., Tarandeau, F., van Tilbeurgh, H., Unger, T., Luna-Vargas, M.P., Velarde, M., Wilmanns, M. & Owens, R.J. Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1103-13. Epub 2006Sep 19. Europe PMC

Combinatorial library approaches for improving soluble protein expression in Escherichia coli. Hart, D.J. & Tarendeau, F. Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):19-26. Epub 2005Dec 14. Europe PMC

An approach to prevent aggregation during the purification and crystallization of wild type acyl coenzyme A: isopenicillin N acyltransferase from Penicillium chrysogenum. Yoshida, H., Hensgens, C.M., van der Laan, J.M., Sutherland, J.D., Hart, D.J. & Dijkstra, B.W. Protein Expr Purif 2005 May;41(1):61-7. Europe PMC

Fabrication of protein function microarrays for systems-oriented proteomic analysis. Blackburn, J.M. & Hart, D.J. Methods Mol Biol. 2005;310:197-216. Europe PMC

Functional protein microarrays for parallel characterisation of p53 mutants. Boutell, J.M., Hart, D.J., Godber, B.L., Kozlowski, R.Z. & Blackburn, J.M. Proteomics 2004 Jul;4(7):1950-8. Europe PMC

A biophysical characterisation of factors controlling dimerisation and selectivity in the NF-kappaB and NFAT families. de Lumley, M., Hart, D.J., Cooper, M.A., Symeonides, S. & Blackburn, J.M. J Mol Biol. 2004 Jun 18;339(5):1059-75. Europe PMC

Distamycin A affects the stability of NF-kappaB p50-DNA complexes in a sequence-dependent manner. Speight, R.E., Hart, D.J. & Blackburn, J.M. J Mol Recognit. 2002 Jan-Feb;15(1):19-26. Europe PMC

Analysis of the NF-kappaB p50 dimer interface by diversity screening. Hart, D.J., Speight, R.E., Sutherland, J.D. & Blackburn, J.M. J Mol Biol 2001 Jul 13;310(3):563-75. Europe PMC

The salt dependence of DNA recognition by NF-kappaB p50: a detailed kinetic analysis of the effects on affinityand specificity. Hart, D.J., Speight, R.E., Cooper, M.A., Sutherland, J.D. & Blackburn, J.M. Nucleic Acids Res. 1999 Feb 15;27(4):1063-9. Europe PMC