Staff at EMBL

Dr. José Márquez

Head of Crystallisation Facility
Márquez Team

EMBL Grenoble, BP 181, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France

Biography

PhD 1997, University of Valencia, Spain.
Postdoctoral research at EMBL.
Staff Scientist at EMBL Grenoble since 2003.
Team Leader since 2005.
Head of Crystallisation Facility since 2012.

Publications

Structure of the MutLalpha C-terminal domain reveals how Mlh1 contributes to Pms1 endonuclease site. Gueneau, E., Dherin, C., Legrand, P., Tellier-Lebegue, C., Gilquin, B., Bonnesoeur, P., Londino, F., Quemener, C., Le Du, M.H., Marquez, J.A., Moutiez, M., Gondry, M., Boiteux, S. & Charbonnier, J.B. Nat Struct Mol Biol. 2013 Apr;20(4):461-8. doi: 10.1038/nsmb.2511. Epub 2013 Feb24. PubMed

CrystalDirect: a new method for automated crystal harvesting based on laser-induced photoablation of thin films. Cipriani, F., Rower, M., Landret, C., Zander, U., Felisaz, F. & Marquez, J.A. Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1393-9. Epub 2012 Sep 18. PubMed

Structural insights into PYR/PYL/RCAR ABA receptors and PP2Cs. Santiago, J., Dupeux, F., Betz, K., Antoni, R., Gonzalez-Guzman, M., Rodriguez, L., Marquez, J.A. & Rodriguez, P.L. Plant Sci. 2012 Jan;182:3-11. Epub 2010 Dec 7. PubMed

A thermal stability assay can help to estimate the crystallization likelihood of biological samples. Dupeux, F., Rower, M., Seroul, G., Blot, D. & Marquez, J.A. Acta Crystallogr D Biol Crystallogr. 2011 Nov;67(Pt 11):915-9. Epub 2011Oct 18. PubMed

A thermodynamic switch modulates abscisic acid receptor sensitivity. Dupeux, F., Santiago, J., Betz, K., Twycross, J., Park, S.Y., Rodriguez, L., Gonzalez-Guzman, M., Jensen, M.R., Krasnogor, N., Blackledge, M., Holdsworth, M., Cutler, S.R., Rodriguez, P.L. & Marquez, J.A. EMBO J. 2011 Aug 16;30(20):4171-84. doi: 10.1038/emboj.2011.294. PubMed

Structural characterization of filaments formed by human Xrcc4-Cernunnos/XLF complex involved in nonhomologous DNA end-joining. Ropars, V., Drevet, P., Legrand, P., Baconnais, S., Amram, J., Faure, G., Marquez, J.A., Pietrement, O., Guerois, R., Callebaut, I., Le Cam, E., Revy, P., de Villartay, J.P. & Charbonnier, J.B. Proc Natl Acad Sci U S A. 2011 Aug 2;108(31):12663-8. doi:10.1073/pnas.1100758108. Epub 2011 Jul 18. PubMed

Modulation of abscisic acid signaling in vivo by an engineered receptor-insensitive protein phosphatase type 2C allele. Dupeux, F., Antoni, R., Betz, K., Santiago, J., Gonzalez-Guzman, M., Rodriguez, L., Rubio, S., Park, S.Y., Cutler, S.R., Rodriguez, P.L. & Marquez, J.A. Plant Physiol. 2011 May;156(1):106-16. Epub 2011 Feb 28. PubMed

Atomic structure of a nanobody-trapped domain-swapped dimer of an amyloidogenic {beta}2-microglobulin variant. Domanska, K., Vanderhaegen, S., Srinivasan, V., Pardon, E., Dupeux, F., Marquez, J.A., Giorgetti, S., Stoppini, M., Wyns, L., Bellotti, V. & Steyaert, J. Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1314-9. Epub 2011 Jan 10. PubMed

The abscisic acid receptor PYR1 in complex with abscisic acid. Santiago, J., Dupeux, F., Round, A., Antoni, R., Park, S.Y., Jamin, M., Cutler, S.R., Rodriguez, P.L. & Marquez, J.A. Nature. 2009 Dec 3;462(7273):665-8. Epub 2009 Nov 8. PubMed

Modulation of drought resistance by the abscisic acid receptor PYL5 through inhibition of clade A PP2Cs. Santiago, J., Rodrigues, A., Saez, A., Rubio, S., Antoni, R., Dupeux, F., Park, S.Y., Marquez, J.A., Cutler, S.R. & Rodriguez, P.L. Plant J. 2009 Nov;60(4):575-88. Epub 2009 Jul 16. PubMed

The crystal structure of the extracellular domain of the inhibitor receptor expressed on myeloid cells IREM-1. Marquez, J.A., Galfre, E., Dupeux, F., Flot, D., Moran, O. & Dimasi, N. J Mol Biol. 2007 Mar 23;367(2):310-8. Epub 2007 Jan 10. PubMed

Expression, crystallization and X-ray data collection from microcrystals of the extracellular domain of the human inhibitory receptor expressed on myeloid cells IREM-1. Dimasi, N., Flot, D., Dupeux, F. & Marquez, J.A. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt3):204-8. Epub 2007 Feb 13. PubMed

Plastic-embedded protein crystals. Ravelli, R.B., Haselmann-Weiss, U., McGeehan, J.E., McCarthy, A.A., Marquez, J.A., Antony, C., Frangakis, A.S. & Stranzl, G. J Synchrotron Radiat. 2007 Jan 1;14(Pt 1):128-32. Epub 2006 Dec 15. PubMed

Structure of the full-length enzyme I of the phosphoenolpyruvate-dependent sugar phosphotransferase system. Marquez, J., Reinelt, S., Koch, B., Engelmann, R., Hengstenberg, W. & Scheffzek, K. J Biol Chem. 2006 Oct 27;281(43):32508-15. Epub 2006 Jul 25. PubMed

The invertase inhibitor Nt-CIF from tobacco: a highly thermostable four-helix bundle with an unusual N-terminal extension. Hothorn, M., D'Angelo, I., Marquez, J.A., Greiner, S. & Scheffzek, K. J Mol Biol 2004 Jan 23;335(4):987-95. PubMed

Conformation of full-length Bruton tyrosine kinase (Btk) from synchrotron X-ray solution scattering. Marquez, J.A., Smith, C.I., Petoukhov, M.V., Lo Surdo, P., Mattsson, P.T., Knekt, M., Westlund, A., Scheffzek, K., Saraste, M. & Svergun, D.I. EMBO J 2003 Sep 15;22(18):4616-24. PubMed

Pyrophosphate-producing protein dephosphorylation by HPr kinase/phosphorylase: a relic of early life? Mijakovic, I., Poncet, S., Galinier, A., Monedero, V., Fieulaine, S., Janin, J., Nessler, S., Marquez, J.A., Scheffzek, K., Hasenbein, S., Hengstenberg, W. & Deutscher, J. Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13442-7. Epub 2002 Oct 1. PubMed

Evolutionary relationship between the bacterial HPr kinase and the ubiquitous PEP-carboxykinase: expanding the P-loop nucleotidyl transferase superfamily. Russell, R.B., Marquez, J.A., Hengstenberg, W. & Scheffzek, K. FEBS Lett. 2002 Apr 24;517(1-3):1-6. PubMed

Structure of the full-length HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution: Mimicking the product/substrate of the phospho transfer reactions. Marquez, J.A., Hasenbein, S., Koch, B., Fieulaine, S., Nessler, S., Russell, R.B., Hengstenberg, W. & Scheffzek, K. Proc Natl Acad Sci U S A 2002 Mar 19;99(6):3458-63. PubMed

Pleckstrin homology (PH) domains. Marquez, J.A. & Saraste, M. In "Encyclopedia of Molecular Medicine," Creighton, T. (Ed.), John Wiley & Sons, New York