Staff at EMBL

Dr. Christiane Schaffitzel

Team Leader
Schaffitzel Team

EMBL Grenoble, BP 181, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France

Biography

PhD 2001, University of Zürich, Switzerland.
Habilitation 2008, ETH Zürich, Switzerland.
Team leader at EMBL Grenoble since 2007.
ERC Investigator

Publications

The architecture of human general transcription factor TFIID core complex. Bieniossek, C., Papai, G., Schaffitzel, C., Garzoni, F., Chaillet, M., Scheer, E., Papadopoulos, P., Tora, L., Schultz, P. & Berger, I. Nature. 2013 Jan 31;493(7434):699-702. doi: 10.1038/nature11791. Epub 2013 Jan 6. PubMed

Low-resolution structure determination of Na(+)-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae by ab initio phasing and electron microscopy. Lunin, V.Y., Lunina, N.L., Casutt, M.S., Knoops, K., Schaffitzel, C., Steuber, J., Fritz, G. & Baumstark, M.W. Acta Crystallogr D Biol Crystallogr. 2012 Jun;68(Pt 6):724-31. Epub 2012 May 17. PubMed

Cryo-electron microscopy of ribosomal complexes in cotranslational folding, targeting, and translocation. Knoops, K., Schoehn, G. & Schaffitzel, C. Wiley Interdiscip. Rev. RNA 3, 429-441. PubMed

Robots, pipelines, polyproteins: Enabling multiprotein expression in prokaryotic and eukaryotic cells. Vijayachandran, L.S., Viola, C., Garzoni, F., Trowitzsch, S., Bieniossek, C., Chaillet, M., Schaffitzel, C., Busso, D., Romier, C., Poterszman, A., Richmond, T.J. & Berger, I. J Struct Biol. 2011 Mar 17. PubMed

Escherichia coli Cotranslational Targeting and Translocation. Von Loeffelholz, O., Botte, M. & Schaffitzel, C. (2010) In: ENCYCLOPEDIA OF LIFE SCIENCES, John Wiley & Sons, Ltd: Chichester, DOI: 10.1002/9780470015902.a0023170.

Structure of the E. coli Co-translational Targeting Complex in the Stable Early Conformation (2011). Estrozi, L.F., Boehringer, D., Shan, S.O., Ban, N. & Schaffitzel, C. Nat. Struct. Mol. Biol. 18, 88-90.

Probing telomeric G-quadruplex DNA structures in cells with in vitro generated single-chain antibody fragments. Schaffitzel, C., Postberg, J., Paeschke, K. & Lipps, H.J. Methods Mol Biol. 2010;608:159-81. PubMed

Automated unrestricted multigene recombineering for multiprotein complex production. Bieniossek, C., Nie, Y., Frey, D., Olieric, N., Schaffitzel, C., Collinson, I., Romier, C., Berger, P., Richmond, T.J., Steinmetz, M.O. & Berger, I. Nat Methods. 2009 Jun;6(6):447-50. Epub 2009 May 3. PubMed

YidC and Oxa1 form dimeric insertion pores on the translating ribosome. Kohler, R., Boehringer, D., Greber, B., Bingel-Erlenmeyer, R., Collinson, I., Schaffitzel, C. & Ban, N. Mol Cell. 2009 May 15;34(3):344-53. PubMed

Recycling of aborted ribosomal 50S subunit-nascent chain-tRNA complexes by the heat shock protein Hsp15. Jiang, L., Schaffitzel, C., Bingel-Erlenmeyer, R., Ban, N., Korber, P., Koning, R.I., de Geus, D.C., Plaisier, J.R. & Abrahams, J.P. J Mol Biol. 2009 Mar 13;386(5):1357-67. Epub 2008 Nov 5. PubMed

Multiple conformational switches in a GTPase complex control co-translational protein targeting. Zhang, X., Schaffitzel, C., Ban, N. & Shan, S.O. Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):1754-9. Epub 2009 Jan 27. PubMed

ACEMBLing multigene expression vectors by recombineering. Nie, Y., Bieniossek, C., Frey, D., Olieric, N., Schaffitzel, C., Steinmetz, M.O. & Berger, I. Nat. Protocols 2009

Molecular mechanism and structure of Trigger Factor bound to the translating ribosome. Merz, F., Boehringer, D., Schaffitzel, C., Preissler, S., Hoffmann, A., Maier, T., Rutkowska, A., Lozza, J., Ban, N., Bukau, B. & Deuerling, E. EMBO J. 2008 Jun 4;27(11):1622-32. Epub 2008 May 22. PubMed

A peptide deformylase-ribosome complex reveals mechanism of nascent chain processing. Bingel-Erlenmeyer, R., Kohler, R., Kramer, G., Sandikci, A., Antolic, S., Maier, T., Schaffitzel, C., Wiedmann, B., Bukau, B. & Ban, N. Nature. 2008 Feb 20;. PubMed

Dynamics of trigger factor interaction with translating ribosomes. Rutkowska, A., Mayer, M.P., Hoffmann, A., Merz, F., Zachmann-Brand, B., Schaffitzel, C., Ban, N., Deuerling, E. & Bukau, B. J Biol Chem. 2008 Feb 15;283(7):4124-32. Epub 2007 Nov 28. PubMed

Transport of Na(+) and K (+) by an antiporter-related subunit from the Escherichia coli NADH dehydrogenase I produced in Saccharomyces cerevisiae. Gemperli, A.C., Schaffitzel, C., Jakob, C. & Steuber, J. Arch Microbiol. 2007 Nov;188(5):509-21. Epub 2007 Jun 22. PubMed

Generation of ribosome nascent chain complexes for structural and functional studies. Schaffitzel, C. & Ban, N. J Struct Biol. 2007 Jun;158(3):463-71. Epub 2007 Jan 23. PubMed

Multiprotein expression strategy for structural biology of eukaryotic complexes. Fitzgerald, D.J., Schaffitzel, C., Berger, P., Wellinger, R., Bieniossek, C., Richmond, T.J. & Berger, I. Structure. 2007 Mar;15(3):275-9. PubMed

Nucleic Acid Science - The Excitement of Discovery. Annual Symposium of the Chemical Society Zurich. Berger, I., Schaffitzel, C. & Bieniossek, C. Chimia 2007 61 837-841

Protein complex expression by using multigene baculoviral vectors. Fitzgerald, D.J., Berger, P., Schaffitzel, C., Yamada, K., Richmond, T.J. & Berger, I. Nat Methods. 2006 Dec;3(12):1021-32. PubMed

Structure of the E. coli signal recognition particle bound to a translating ribosome. Schaffitzel, C., Oswald, M., Berger, I., Ishikawa, T., Abrahams, J.P., Koerten, H.K., Koning, R.I. & Ban, N. Nature. 2006 Nov 23;444(7118):503-6. Epub 2006 Oct 29. PubMed

Elongation arrest by SecM via a cascade of ribosomal RNA rearrangements. Mitra, K., Schaffitzel, C., Fabiola, F., Chapman, M.S., Ban, N. & Frank, J. Mol Cell. 2006 May 19;22(4):533-43. PubMed

Multi-level regulation of myotubularin-related protein-2 phosphatase activity by myotubularin-related protein-13/set-binding factor-2. Berger, P., Berger, I., Schaffitzel, C., Tersar, K., Volkmer, B. & Suter, U. Hum Mol Genet. 2006 Feb 15;15(4):569-79. Epub 2006 Jan 6. PubMed

Ribosome, high resolution structure and function. C. Schaffitzel & N. Ban In "Proteins: From Analytics to Structural Genomics", R. Meyers (ed), Wiley-VCH, Weinheim, 2006, Vol. 1, Chapter 17, 487-514

Structure of the E. coli protein-conducting channel bound to a translating ribosome. Mitra, K., Schaffitzel, C., Shaikh, T., Tama, F., Jenni, S., Brooks CL, 3rd, Ban, N. & Frank, J. Nature. 2005 Nov 17;438(7066):318-24. PubMed

In vitro Selection and Evolution of Protein-Ligand Interactions by Ribosome Display. C. Schaffitzel, C. Zahnd, P. Amstutz, B. Luginbühl & A. Plückthun In "Protein-Protein Interactions: A Molecular Cloning Manual", 2nd edition, E. Golemis & P. Adams (eds), 2005, Cold Spring Harbor Laboratory Press, New York.

The ribosome, high resolution structure and function. C. Schaffitzel & N. Ban In "Encyclopedia of Molecular Cell Biology and Molecular Medicine", 2nd edition. R. Meyers (ed), Wiley-VCH, Weinheim, 2005, Vol. 12, pp.483-512

Structural Basis of Protein Synthesis. C. Schaffitzel & N. Ban In "Supramolecular Structure and Function 8", G. Prifat-Mryljak (ed), Kluwer Academic/Plenum Publishers, New York, 2004, Chapter 1, 1-13.

Membrane association of myotubularin-related protein 2 is mediated by a pleckstrin homology-GRAM domain and a coiled-coil dimerization module. Berger, P., Schaffitzel, C., Berger, I., Ban, N. & Suter, U. Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12177-82. Epub 2003 Oct 6. PubMed

Direct interaction of Ca2+/calmodulin inhibits histone deacetylase 5 repressor core binding to myocyte enhancer factor 2. Berger, I., Bieniossek, C., Schaffitzel, C., Hassler, M., Santelli, E. & Richmond, T.J. J Biol Chem. 2003 May 16;278(20):17625-35. Epub 2003 Mar 6. PubMed

Organization of the macronuclear gene-sized pieces of stichotrichous ciliates into a higher order structure via telomere-matrix interactions. Jonsson, F., Postberg, J., Schaffitzel, C. & Lipps, H.J. Chromosome Res. 2002;10(6):445-53. PubMed

Protein-fold evolution in the test tube. Schaffitzel, C. & Pluckthun, A. Trends Biochem Sci. 2001 Oct;26(10):577-9. PubMed

In vitro generated antibodies specific for telomeric guanine-quadruplex DNA react with Stylonychia lemnae macronuclei. Schaffitzel, C., Berger, I., Postberg, J., Hanes, J., Lipps, H.J. & Pluckthun, A. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8572-7. Epub 2001 Jul 3. PubMed

Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display. Hanes, J., Schaffitzel, C., Knappik, A. & Pluckthun, A. Nat Biotechnol. 2000 Dec;18(12):1287-92. PubMed

In vitro selection and evolution of proteins. Pluckthun, A., Schaffitzel, C., Hanes, J. & Jermutus, L. Adv Protein Chem. 2000;55:367-403. PubMed

Ribosome display: an in vitro method for selection and evolution of antibodies from libraries. Schaffitzel, C., Hanes, J., Jermutus, L. & Pluckthun, A. J Immunol Methods. 1999 Dec 10;231(1-2):119-35. PubMed

Comparison of Escherichia coli and rabbit reticulocyte ribosome display systems. Hanes, J., Jermutus, L., Schaffitzel, C. & Pluckthun, A. FEBS Lett. 1999 Apr 30;450(1-2):105-10. PubMed

Identification of an Na+-dependent malonate transporter of Malonomonas rubra and its dependence on two separate genes. Schaffitzel, C., Berg, M., Dimroth, P. & Pos, K.M. J Bacteriol. 1998 May;180(10):2689-93. PubMed