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PublicationsSchaffitzel Team

Total: 43 publication(s)


Muras V, Claussen B, Karuppasamy M, Schaffitzel C, Steuber J. (2014)
Continuous fluorescence-based measurement of redox-driven sodium ion translocation.
Anal. Biochem. 459:53-55. doi:10.1016/j.ab.2014.05.012
Europe PMC | doi

Schulze RJ, Komar J, Botte M, Allen WJ, Whitehouse S, Gold VA, Lycklama A Nijeholt JA, Huard K, Berger I, Schaffitzel C, Collinson I. (2014)
Membrane protein insertion and proton-motive-force-dependent secretion through the bacterial holo-translocon SecYEG-SecDF-YajC-YidC
Proc. Natl. Acad. Sci. U.S.A. 111(13):4844-4849. doi:10.1073/pnas.1315901111
Europe PMC | doi

Clerici M, Deniaud A, Boehm V, Gehring NH, Schaffitzel C, Cusack S. (2014)
Structural and functional analysis of the three MIF4G domains of nonsense-mediated decay factor UPF2
Nucleic Acids Res. 42(4):2673-2686. doi:10.1093/nar/gkt1197
Europe PMC | doi


Mesa P, Deniaud A, Montoya G, Schaffitzel C. (2013)
Directly from the source: endogenous preparations of molecular machines
Curr. Opin. Struct. Biol. 23(3):319-325. doi:10.1016/
Europe PMC | doi

von Loeffelholz O, Knoops K, Ariosa A, Zhang X, Karuppasamy M, Huard K, Schoehn G, Berger I, Shan SO, Schaffitzel C. (2013)
Structural basis of signal sequence surveillance and selection by the SRP-FtsY complex
Nat. Struct. Mol. Biol. 20(5):604-610. doi:10.1038/nsmb.2546
Europe PMC | doi

Bieniossek C, Papai G, Schaffitzel C, Garzoni F, Chaillet M, Scheer E, Papadopoulos P, Tora L, Schultz P, Berger I. (2013)
The architecture of human general transcription factor TFIID core complex
Nature 493(7434):699-702. doi:10.1038/nature11791
Europe PMC | doi


Lunin VY, Lunina NL, Casutt MS, Knoops K, Schaffitzel C, Steuber J, Fritz G, Baumstark MW. (2012)
Low-resolution structure determination of Na(+)-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae by ab initio phasing and electron microscopy
Acta Crystallogr. D Biol. Crystallogr. 68(Pt 6):724-731. doi:10.1107/s0907444912012012
Europe PMC | doi

Knoops K, Schoehn G, Schaffitzel C. (2012)
Cryo-electron microscopy of ribosomal complexes in cotranslational folding, targeting, and translocation
Wiley Interdiscip Rev RNA 3(3):429-441. doi:10.1002/wrna.119
Europe PMC | doi


Vijayachandran LS, Viola C, Garzoni F, Trowitzsch S, Bieniossek C, Chaillet M, Schaffitzel C, Busso D, Romier C, Poterszman A, Richmond TJ, Berger I. (2011)
Robots, pipelines, polyproteins: enabling multiprotein expression in prokaryotic and eukaryotic cells
J. Struct. Biol. 175(2):198-208. doi:10.1016/j.jsb.2011.03.007
Europe PMC | doi

Estrozi LF, Boehringer D, Shan SO, Ban N, Schaffitzel C. (2011)
Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor
Nat. Struct. Mol. Biol. 18(1):88-90. doi:10.1038/nsmb.1952
Europe PMC | doi

von Loeffelholz O, Botte M, Schaffitzel C. (2011)
Escherichia coli cotranslational targeting and translocation
Europe PMC | doi


Schaffitzel C, Postberg J, Paeschke K, Lipps HJ. (2010)
Probing telomeric G-quadruplex DNA structures in cells with in vitro generated single-chain antibody fragments
Methods Mol. Biol. 608:159-181. doi:10.1007/978-1-59745-363-9_11
Europe PMC | doi


Bieniossek C, Nie Y, Frey D, Olieric N, Schaffitzel C, Collinson I, Romier C, Berger P, Richmond TJ, Steinmetz MO, Berger I. (2009)
Automated unrestricted multigene recombineering for multiprotein complex production
Nat. Methods 6(6):447-450. doi:10.1038/nmeth.1326
Europe PMC | doi

Nie Y, Bieniossek C, Frey D, Olieric N, Schaffitzel C. (2009)
ACEMBLing multigene expression vectors by recombineering
Protocol Exchange doi:10.1038/nprot.2009.104
Europe PMC | doi

Kohler R, Boehringer D, Greber B, Bingel-Erlenmeyer R, Collinson I, Schaffitzel C, Ban N. (2009)
YidC and Oxa1 form dimeric insertion pores on the translating ribosome
Mol. Cell 34(3):344-353. doi:10.1016/j.molcel.2009.04.019
Europe PMC | doi

Jiang L, Schaffitzel C, Bingel-Erlenmeyer R, Ban N, Korber P, Koning RI, de Geus DC, Plaisier JR, Abrahams JP. (2009)
Recycling of aborted ribosomal 50S subunit-nascent chain-tRNA complexes by the heat shock protein Hsp15
J. Mol. Biol. 386(5):1357-1367. doi:10.1016/j.jmb.2008.10.079
Europe PMC | doi

Zhang X, Schaffitzel C, Ban N, Shan SO. (2009)
Multiple conformational switches in a GTPase complex control co-translational protein targeting
Proc. Natl. Acad. Sci. U.S.A. 106(6):1754-1759. doi:10.1073/pnas.0808573106
Europe PMC | doi


Merz F, Boehringer D, Schaffitzel C, Preissler S, Hoffmann A, Maier T, Rutkowska A, Lozza J, Ban N, Bukau B, Deuerling E. (2008)
Molecular mechanism and structure of Trigger Factor bound to the translating ribosome
EMBO J. 27(11):1622-1632. doi:10.1038/emboj.2008.89
Europe PMC | doi

Bingel-Erlenmeyer R, Kohler R, Kramer G, Sandikci A, Antolić S, Maier T, Schaffitzel C, Wiedmann B, Bukau B, Ban N. (2008)
A peptide deformylase-ribosome complex reveals mechanism of nascent chain processing
Nature 452(7183):108-111. doi:10.1038/nature06683
Europe PMC | doi

Rutkowska A, Mayer MP, Hoffmann A, Merz F, Zachmann-Brand B, Schaffitzel C, Ban N, Deuerling E, Bukau B. (2008)
Dynamics of trigger factor interaction with translating ribosomes
J. Biol. Chem. 283(7):4124-4132. doi:10.1074/jbc.m708294200
Europe PMC | doi


Gemperli AC, Schaffitzel C, Jakob C, Steuber J. (2007)
Transport of Na(+) and K (+) by an antiporter-related subunit from the Escherichia coli NADH dehydrogenase I produced in Saccharomyces cerevisiae
Arch. Microbiol. 188(5):509-521. doi:10.1007/s00203-007-0272-3
Europe PMC | doi

Schaffitzel C, Ban N. (2007)
Generation of ribosome nascent chain complexes for structural and functional studies
J. Struct. Biol. 158(3):463-471. doi:10.1016/j.jsb.2007.01.005
Europe PMC | doi

Fitzgerald DJ, Schaffitzel C, Berger P, Wellinger R, Bieniossek C, Richmond TJ, Berger I. (2007)
Multiprotein expression strategy for structural biology of eukaryotic complexes
Structure 15(3):275-279. doi:10.1016/j.str.2007.01.016
Europe PMC | doi

Berger I, Schaffitzel C, Bieniossek C. (2007)
Nucleic Acid Science – The Excitement of Discovery: Annual Symposium of the Chemical Society Zurich CGZ, Zurich, October 26, 2007
Chimia (Aarau) 61(12):837-841.
Europe PMC


Schaffitzel C, Ban N. (2006)
Ribosome, High Resolution Structure and Function

Fitzgerald DJ, Berger P, Schaffitzel C, Yamada K, Richmond TJ, Berger I. (2006)
Protein complex expression by using multigene baculoviral vectors
Nat. Methods 3(12):1021-1032. doi:10.1038/nmeth983
Europe PMC | doi

Schaffitzel C, Oswald M, Berger I, Ishikawa T, Abrahams JP, Koerten HK, Koning RI, Ban N. (2006)
Structure of the E. coli signal recognition particle bound to a translating ribosome
Nature 444(7118):503-506. doi:10.1038/nature05182
Europe PMC | doi

Schaffitzel C, Ban N. (2006)
Ribosome, high resolution structure and function

Mitra K, Schaffitzel C, Fabiola F, Chapman MS, Ban N, Frank J. (2006)
Elongation arrest by SecM via a cascade of ribosomal RNA rearrangements
Mol. Cell 22(4):533-543. doi:10.1016/j.molcel.2006.05.003
Europe PMC | doi

Berger P, Berger I, Schaffitzel C, Tersar K, Volkmer B, Suter U. (2006)
Multi-level regulation of myotubularin-related protein-2 phosphatase activity by myotubularin-related protein-13/set-binding factor-2
Hum. Mol. Genet. 15(4):569-579. doi:10.1093/hmg/ddi473
Europe PMC | doi


Mitra K, Schaffitzel C, Shaikh T, Tama F, Jenni S, Brooks CL 3rd, Ban N, Frank J. (2005)
Structure of the E. coli protein-conducting channel bound to a translating ribosome
Nature 438(7066):318-324. doi:10.1038/nature04133
Europe PMC | doi

Schaffitzel C, Zahnd C, Amstutz P, Luginbühl B, Plückthun A. (2005)
In vitro Selection and Evolution of Protein-Ligand Interactions by Ribosome Display


Schaffitzel C, Ban N. (2004)
Structural Basis of Protein Synthesis


Berger P, Schaffitzel C, Berger I, Ban N, Suter U. (2003)
Membrane association of myotubularin-related protein 2 is mediated by a pleckstrin homology-GRAM domain and a coiled-coil dimerization module
Proc. Natl. Acad. Sci. U.S.A. 100(21):12177-12182. doi:10.1073/pnas.2132732100
Europe PMC | doi

Berger I, Bieniossek C, Schaffitzel C, Hassler M, Santelli E, Richmond TJ. (2003)
Direct interaction of Ca2+/calmodulin inhibits histone deacetylase 5 repressor core binding to myocyte enhancer factor 2
J. Biol. Chem. 278(20):17625-17635. doi:10.1074/jbc.m301646200
Europe PMC | doi


Jönsson F, Postberg J, Schaffitzel C, Lipps HJ. (2002)
Organization of the macronuclear gene-sized pieces of stichotrichous ciliates into a higher order structure via telomere-matrix interactions
Chromosome Res. 10(6):445-453. doi:10.1023/a:1021065512933
Europe PMC | doi


Schaffitzel C, Plückthun A. (2001)
Protein-fold evolution in the test tube
Trends Biochem. Sci. 26(10):577-579. doi:10.1016/s0968-0004(01)01959-4
Europe PMC | doi

Schaffitzel C, Berger I, Postberg J, Hanes J, Lipps HJ, Plückthun A. (2001)
In vitro generated antibodies specific for telomeric guanine-quadruplex DNA react with Stylonychia lemnae macronuclei
Proc. Natl. Acad. Sci. U.S.A. 98(15):8572-8577. doi:10.1073/pnas.141229498
Europe PMC | doi


Hanes J, Schaffitzel C, Knappik A, Plückthun A. (2000)
Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display
Nat. Biotechnol. 18(12):1287-1292. doi:10.1038/82407
Europe PMC | doi

Plückthun A, Schaffitzel C, Hanes J, Jermutus L. (2000)
In vitro selection and evolution of proteins
Adv. Protein Chem. 55:367-403. doi:10.1016/s0065-3233(01)55009-3
Europe PMC | doi


Schaffitzel C, Hanes J, Jermutus L, Plückthun A. (1999)
Ribosome display: an in vitro method for selection and evolution of antibodies from libraries
J. Immunol. Methods 231(1-2):119-135. doi:10.1016/s0022-1759(99)00149-0
Europe PMC | doi

Hanes J, Jermutus L, Schaffitzel C, Plückthun A. (1999)
Comparison of Escherichia coli and rabbit reticulocyte ribosome display systems
FEBS Lett. 450(1-2):105-110. doi:10.1016/s0014-5793(99)00475-5
Europe PMC | doi


Schaffitzel C, Berg M, Dimroth P, Pos KM. (1998)
Identification of an Na+-dependent malonate transporter of Malonomonas rubra and its dependence on two separate genes
J. Bacteriol. 180(10):2689-2693.
Europe PMC