Eukaryotic Expression Facility

Purpose of the platform (operated by EMBL)

Eukaryotic Expression Facility EEF

The EEF is an EMBL installation in the CIBB building which uses MultiBac-EMBL, our state-of-the-art baculovirus expression system for multiprotein complex production developed by the Berger Group.

• Introduction into baculovirus/insect cell expression
• Introduction into our MultiBac-EMBL system for multiprotein complex production
• Multigene expression cassette generatio
• Composite baculovirus generation
• Virus generation and amplification
• Viral performance and protein production monitoring
• Smale-, medium-, large scale protein (complex) expression

Courtesy of Sahlmueller, Bruegger and Wieland