Florent Cipriani
EMBL Grenoble, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France

Tel: +33 47620-7599
Email: cipriani@embl.fr

The Flex robotics family

The Flex robotics family

Designed to handle sample holders used in Macromolecular X-ray crystallography, the Flex robotics family is built around a 6 axis industrial robot. The family currently includes 3 members equipped with different storage Dewars. They are all provided with a tool changer and various handling tools.

FlexED8 (picture), a sample changer compatible with SPINE, NewPin and miniSpine sample holders that can hold up to 252 sample holders in a small footprint open Dewar with built in ice filtering system. Different cryo-grippers are used to safely transfer the sample holders to a goniometer. The sample exchange time can be as low as 4 seconds when the Twin SPINE cryo-gripper is used. FlexED8 was developed in the context of the NewPin project and tested on the EMBL/ESRF/INDIA BM14 CRG beamline.

FlexHCD, a sample changer compatible with SPINE sample holders. It includes a modified High Capacity Dewar initially developed within the ESRF MX group. It can hold up to 384 samples in uni-pucks or 240 samples with vials in SC3 pucks, or a mix of the two. The sample holders in uni-pucks are transferred using a single or a Twin cryo-gripper and with the IRELEC-Alcen flipping gripper when stored with vials in SC3 pucks. FlexHCD is installed at the ESRF ID30B beamline and being duplicated to equip four other ESRF MX beamlines.

FlexED3, a cryo-storage unit associated to a CrystalDirect™ (see below) harvester to automatically store crystals harvested with SPINE sample holders in SC3 pucks. FlexED3 is currently in operation at the Grenoble HTX lab.

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The CrystalDirect harvesting station

The CrystalDirect harvesting station

Operated by the HTX lab since 2017, it allows to automatically harvest, vitrify and mount on SPINE sample holders crystals of any size, as well as micro-crystals harvested in batches.With a storage capacity of several hundred samples in SC3 pucks or in uni-pucks and a turnover time of 1'30", it is extensively used in fragment screening applications.

Inside look

From a collaboration with the High throughput crystallization team (J.A. Marquez)

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CrystalDirect™

CrystalDirect™

Jointly with the EMBL Grenoble HTX Team, J A Marquez.

Automated crystal harvesting and in-plate crystal screening.

Download the 'CrystalDirect Laser Harvesting' video [9.6MB: right click, 'download/save link as']

Download the 'In-Situ data collection' video [12.4MB: right click, 'download/save link as'] – EMBL/ESRF BM14 beamline, Hassan Belrhali

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MD3 vertical Kappa Diffractometer

MD3 vertical Kappa Diffractometer

In collaboration with EMBL@PETRA-III MX2, G Bourenkov and ARINAX.

Download 'MD3 SOC-1' video [508KB: right click, 'download/save link as'] – Kappa = 0°, Omega 160°/s; Glass needle 0.3 µm tip; Scale 1 µm/div

Download 'MD3 SOC-2' video [8.1MB: right click, 'download/save link as'] – Kappa = 48°, Omega 45°/sec; Glass needle 1 µm hole; Scale 10 µm/div

Download 'MD3 4D scan' video [4.1MB: right click, 'download/save link as'] – Needle Ø8 µm X 150 µm; 4D scan: 180°, 18°/s; Scale 10 µm/div

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HC1 Crystal Humidity Control

HC1 Crystal Humidity Control
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Sample Changer SC3

Sample Changer SC3

Download 'Load/unload one sample (real-time)' video [3.5MB: right click, 'download/save link as']

ESRF  ID14-1, ID14-2, ID14-3, ID14-4, ID29, ID23-1, ID23-2; embl-France  ESRF BM14

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Mini Kappa Goniometer Head

Mini Kappa Goniometer Head

 

Download 'MK2 - mini Kappa goniometer head' details [225KB]

Download 'MK2 - mini Kappa goniometer head' video 1 [3.4MB: right click, 'download/ save link as'] – stability when mounted on a MD2 diffractometer @ Chi = 0, Phi fixed, Omega 90 deg/s. Grid 10 µm/div; vertical arrow 4 µm

Download 'MK2 - mini Kappa goniometer head' video 2 [5.2MB: right click, 'download/ save link as'] – stability when mounted on a MD2 diffractometer @ Chi = 48 deg, Phi fixed, Omega 20 deg/s ... 90 deg/s. Grid 10 µm/div; horizontal arrow 5 µm

ESRF  ID14-4, ID29, ID23-1; embl-France  ESRF BM14 CRG, ESRF BM30  CEA-IBS CRG, APS  LS CAT; Diamond; ALS; Bessy

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MD2M Diffractometer (minidiffractometer)

MD2M Diffractometer (minidiffractometer)

Download 'MD2M - MicroDiffractometer' details [303KB]

ESRF  ID14-1, ID14-2, ID14-4, ID29, ID23-1, ID23-2

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Microspectrophotometer

Microspectrophotometer

Download 'MicroSpecrophotometer lens setup' details [897KB]

Use it at the ESRF

ESRF ID14-x, DLS

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MD2 Diffractometer (microdiffractometer)

MD2 Diffractometer (microdiffractometer)

Download 'Microdiffractometer MD2' details [1.7MB]

SLS  X06S; ESRF  ID14-3; embl-France  ESRF BM14 CRG; APS NE CAT, LS CAT; Bessy, ALS, CLS, SSRL, EMBL-HH, Alba, MAX-lab, Soleil

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Automatic Crystal Alignment Library C3D

Automatic Crystal Alignment Library C3D

Read more about C3D - a tool for automated crystal alignment on high throughput macromolecular X-ray beamlines - on the EMBLEM Technology transfer website.

ESRF ID14-1, ID14-2, ID14-3, ID14-4, ID29, ID23-1, ID23-2; EMBL/ESRF BM14 (CRG); NSLS

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On Axis Video-microscope

On Axis Video-microscope

Download 'OAV - On beam Axis Video-microscope' details [356KB]

ESRF BM30 FIP CNRS/IBS GRG + EMBL/ESRF BM14 (CRG); NSLS

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Piezoelectric Shutter

Piezoelectric Shutter

Download '1″ exposure, 5 passes' video [336KB: right click, 'download/ save link as'] –­ real-time 5 x 0.2″

ESRF ID14-1, ID14-2, ID14-3, ID14-4, ID29, ID23-1, ID23-2; APS NE-CAT, EMBL-HH

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