EMBL Grenoble, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France
Tel: +33 47620-7599
The Flex robotics family
Designed to handle sample holders used in Macromolecular X-ray crystallography, the Flex robotics family is built around a 6 axis industrial robot. The family currently includes 3 members equipped with different storage Dewars. They are all provided with a tool changer and various handling tools.
FlexED8 (picture), a sample changer compatible with SPINE, NewPin and miniSpine sample holders that can hold up to 252 sample holders in a small footprint open Dewar with built in ice filtering system. Different cryo-grippers are used to safely transfer the sample holders to a goniometer. The sample exchange time can be as low as 4 seconds when the Twin SPINE cryo-gripper is used. FlexED8 was developed in the context of the NewPin project and tested on the EMBL/ESRF/INDIA BM14 CRG beamline.
FlexHCD, a sample changer compatible with SPINE sample holders. It includes a modified High Capacity Dewar initially developed within the ESRF MX group. It can hold up to 384 samples in uni-pucks or 240 samples with vials in SC3 pucks, or a mix of the two. The sample holders in uni-pucks are transferred using a single or a Twin cryo-gripper and with the IRELEC-Alcen flipping gripper when stored with vials in SC3 pucks. FlexHCD is installed at the ESRF ID30B beamline and being duplicated to equip four other ESRF MX beamlines.
FlexED3, a cryo-storage unit associated to a CrystalDirect™ (see below) harvester to automatically store crystals harvested with SPINE sample holders in SC3 pucks. FlexED3 is currently in operation at the Grenoble HTX lab.
The CrystalDirect harvesting station
Operated by the HTX lab since 2017, it allows to automatically harvest, vitrify and mount on SPINE sample holders crystals of any size, as well as micro-crystals harvested in batches.With a storage capacity of several hundred samples in SC3 pucks or in uni-pucks and a turnover time of 1'30", it is extensively used in fragment screening applications.
From a collaboration with the High throughput crystallization team (J.A. Marquez)
Jointly with the EMBL Grenoble HTX Team, J A Marquez.
Automated crystal harvesting and in-plate crystal screening.
Download the 'CrystalDirect Laser Harvesting' video [9.6MB: right click, 'download/save link as']
Download the 'In-Situ data collection' video [12.4MB: right click, 'download/save link as'] – EMBL/ESRF BM14 beamline, Hassan Belrhali
NewPin and miniSpine
Tentative for a new European sample holder standard for cryo-crystallography
MD3 vertical Kappa Diffractometer
Download 'MD3 SOC-1' video [508KB: right click, 'download/save link as'] – Kappa = 0°, Omega 160°/s; Glass needle 0.3 µm tip; Scale 1 µm/div
Download 'MD3 SOC-2' video [8.1MB: right click, 'download/save link as'] – Kappa = 48°, Omega 45°/sec; Glass needle 1 µm hole; Scale 10 µm/div
Download 'MD3 4D scan' video [4.1MB: right click, 'download/save link as'] – Needle Ø8 µm X 150 µm; 4D scan: 180°, 18°/s; Scale 10 µm/div
HC1 Crystal Humidity Control
Collaborations: Crystal Dehydration
ESRF, Diamond, MAX-Lab, Bessy, CLS
Sample Changer SC3
Download 'Load/unload one sample (real-time)' video [3.5MB: right click, 'download/save link as']
ESRF ID14-1, ID14-2, ID14-3, ID14-4, ID29, ID23-1, ID23-2; embl-France ESRF BM14
Mini Kappa Goniometer Head
Download 'MK2 - mini Kappa goniometer head' video 1 [3.4MB: right click, 'download/ save link as'] – stability when mounted on a MD2 diffractometer @ Chi = 0, Phi fixed, Omega 90 deg/s. Grid 10 µm/div; vertical arrow 4 µm
Download 'MK2 - mini Kappa goniometer head' video 2 [5.2MB: right click, 'download/ save link as'] – stability when mounted on a MD2 diffractometer @ Chi = 48 deg, Phi fixed, Omega 20 deg/s ... 90 deg/s. Grid 10 µm/div; horizontal arrow 5 µm
ESRF ID14-4, ID29, ID23-1; embl-France ESRF BM14 CRG, ESRF BM30 CEA-IBS CRG, APS LS CAT; Diamond; ALS; Bessy
MD2M Diffractometer (minidiffractometer)
ESRF ID14-1, ID14-2, ID14-4, ID29, ID23-1, ID23-2
Use it at the ESRF
ESRF ID14-x, DLS
MD2 Diffractometer (microdiffractometer)
SLS X06S; ESRF ID14-3; embl-France ESRF BM14 CRG; APS NE CAT, LS CAT; Bessy, ALS, CLS, SSRL, EMBL-HH, Alba, MAX-lab, Soleil
Automatic Crystal Alignment Library C3D
Read more about C3D - a tool for automated crystal alignment on high throughput macromolecular X-ray beamlines - on the EMBLEM Technology transfer website.
ESRF ID14-1, ID14-2, ID14-3, ID14-4, ID29, ID23-1, ID23-2; EMBL/ESRF BM14 (CRG); NSLS
On Axis Video-microscope
ESRF BM30 FIP CNRS/IBS GRG + EMBL/ESRF BM14 (CRG); NSLS
Download '1″ exposure, 5 passes' video [336KB: right click, 'download/ save link as'] – real-time 5 x 0.2″
ESRF ID14-1, ID14-2, ID14-3, ID14-4, ID29, ID23-1, ID23-2; APS NE-CAT, EMBL-HH