EMBL Grenoble, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France
Tel: +33 4 76 20 7951
A project to automate the preparation of Cryo-EM SPA grids with multiple samples on a grid and time resolved freezing. The production of associated nanometer scale 2D ice thickness maps will provide microscope direct access to potentially usable sample areas.
In consultation with electron microscopists of the EMBL Grenoble and Heidelberg sites.
CrystalDirect-to-Beam at MASSIF 1
A project to integrate the CrystalDirect-to-beam concept at MASSIF 1, the future ESRF-EBS fully automated MX beamline ID30A1. Crystals supplied in CrystalDirect plates will be automatically harvested and data collected at cryogenic or at room temperature without manual intervention.
In collaboration with the Synchrotron Crystallography Team (M. Bowler, A. McCarthy), the High Throughput Crystallization team (J.A. Marquez) and the ESRF MX Group (G. Leonard)
The CrystalDirect Harvester 3
Designed to operate in a normal room, its built in temperature control system allows harvesting crystals at 4 and 20 deg Celsius. Two times faster than the previous generation, it offer 30 seconds harvest turnover time.
Download the CDh3 Harvesting video
In collaboration with the High Throughput Crystallization team (J.A. Marquez)
CryoRFID sample identification
An electronic identification system based on Radio Frequency IDentification (RFID) tags compatible with cryogenic temperature (CryoRFID) is being developed to track samples stored in cryo containers, like MX pucks, Cryo-EM pucks or grid boxes. Specific readers are used to read the UIDs of the CryoRFID tags and to store/retrieve user information in the tags.
The MD3upSSX diffractometer
Optimized for serial data collection on solid supports and kinetic studies, it will equip the future ESRF-EBS micro-focus beamline ID29. A fast SSX scan head (up to 100 mm/s) and a synchronization system will allow shooting up to 2000 micro-crystals per second with x-ray photon bunches delivered by the beam chopper of the beamline.
Download the MD3upSSX scan video (50 mm/s line scans)
In collaboration with the ESRF MX group (D.de Sanctis) and the company ARINAX.
ID30B, the ESRF miniSPINE pilot beamline
The ESRF-EBS MX beamline ID30B will see its FlexHCD sample changer equipped with miniSPINE single and double grippers, making it first miniSPINE pilot beamline opened to users.
Download the miniSPINE video
Two new BioSAXS sample exposure units
Two new BioSAXS sample exposure units are developed for the ESRF-EBS beamline ID29 and the EMBL@PETRA-III P13 beamline. The fist, to optimally expose sample solutions in thin glass capillaries, will offer in-situ absorption spectrophotometry and DLS particle size measurement to control the quality of the samples. More flexible, the second is a vacuum chamber with motorized stage and on beam axis camera. It is designed to expose sample solutions in micro-fluidic devices.
In collaboration with the EMBL Hamburg site (D. Svergun, C. Blanchet) and the ESRF (P. Pernot, M. Tully)