EMBL Grenoble, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France

Tel: +33 47620-7061

E-mail: iberger@embl.fr


  • PhD 1995, MIT Cambridge and Leibniz University, Hannover.
    Postdoctoral research at MIT and the Institute of Molecular Biology and Biophysics (IMB), ETH Zürich.
    Habilitation 2005, ETH.
    Group leader at IMB since 2005.
    Group leader at EMBL Grenoble since 2007.
    Full Professor of Biochemistry at University of Bristol, UK, since 2014.
    Wellcome Trust Senior Investigator.


Total: 64 publication(s)


Monteiro F, Bernal V, Chaillet M, Berger I, Alves PM. (2016)


J. Biotechnol. doi: 10.1016/j.jbiotec.2016.06.029
Europe PMC | doi

Komar J, Alvira S, Schulze R, Martin R, Lycklama A Nijeholt J, Lee S, Dafforn T, Deckers-Hebestreit G, Berger I, Schaffitzel C, Collinson I. (2016)

Membrane protein insertion and assembly by the bacterial holo-translocon SecYEG-SecDF-YajC-YidC.

Biochem. J. doi: 10.1042/BCJ20160545
Europe PMC | doi

Swale C, Monod A, Tengo L, Labaronne A, Garzoni F, Bourhis JM, Cusack S, Schoehn G, Berger I, Ruigrok RW, Crépin T. (2016)

Structural characterization of recombinant IAV polymerase reveals a stable complex between viral PA-PB1 heterodimer and host RanBP5.

Sci Rep 6 doi: 10.1038/srep24727
Europe PMC | doi

Nie Y, Chaillet M, Becke C, Haffke M, Pelosse M, Fitzgerald D, Collinson I, Schaffitzel C, Berger I. (2016)

ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts.

Adv. Exp. Med. Biol. 896:27-42. doi: 10.1007/978-3-319-27216-0_3
Europe PMC | doi

Sari D, Gupta K, Raj DB, Aubert A, Drncová P, Garzoni F, Fitzgerald D, Berger I. (2016)

The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics.

Adv. Exp. Med. Biol. 896:199-215. doi: 10.1007/978-3-319-27216-0_13
Europe PMC | doi

Mansouri M, Bellon-Echeverria I, Rizk A, Ehsaei Z, Cianciolo Cosentino C, Silva CS, Xie Y, Boyce FM, Davis MW, Neuhauss SC, Taylor V, Ballmer-Hofer K, Berger I, Berger P. (2016)

Highly efficient baculovirus-mediated multigene delivery in primary cells.

Nat Commun 7 doi: 10.1038/ncomms11529
Europe PMC | doi

Zhang W, Aubert A, Gomez de Segura JM, Karuppasamy M, Basu S, Murthy A, Diamante A, Drury TA, Balmer J, Cramard J, Watson AA, Lando D, Lee SF, Palayret M, Kloet S, Smits A, Deery M, Vermeulen M, Hendrich B, Klenerman D, Schaffitzel C, Berger I, Laue ED. (2016)

The Nucleosome Remodeling and Deacetylase Complex NuRD is built from preformed catalytically active sub-modules.

J. Mol. Biol. doi: 10.1016/j.jmb.2016.04.025
Europe PMC | doi

Gupta K, Sari-Ak D, Haffke M, Trowitzsch S, Berger I. (2016)

Zooming In On Transcription Preinitiation.

J. Mol. Biol. doi: 10.1016/j.jmb.2016.04.003
Europe PMC | doi


Beltran-Sastre V, Benisty H, Burnier J, Berger I, Serrano L, Kiel C. (2015)

Tuneable endogenous mammalian target complementation via multiplexed plasmid-based recombineering.

Sci Rep 5 doi: 10.1038/srep17432
Europe PMC | doi

Riss A, Scheer E, Joint M, Trowitzsch S, Berger I, Tora L. (2015)

Subunits of ADA-Two-A-Containing (ATAC) or Spt-Ada-Gcn5-Acetyltrasferase (SAGA) Coactivator Complexes Enhance the Acetyltransferase Activity of GCN5.

J Biol Chem doi: 10.1074/jbc.M115.668533
Europe PMC | doi

Berger I, Poterszman A. (2015)

Baculovirus expression: old dog, new tricks.

Bioengineered 6(6):316-322. doi: 10.1080/21655979.2015.1104433
Europe PMC | doi

Berger I, Bill RM. (2015)

Editorial overview: Complex expressions: New protein production tools for structural biology.

Curr. Opin. Struct. Biol. doi: 10.1016/j.sbi.2015.05.006
Europe PMC | doi

Crépin T, Swale C, Monod A, Garzoni F, Chaillet M, Berger I. (2015)

Polyproteins in structural biology.

Curr. Opin. Struct. Biol. 32:139-146. doi: 10.1016/j.sbi.2015.04.007
Europe PMC | doi

Komar J, Botte M, Collinson I, Schaffitzel C, Berger I. (2015)

ACEMBLing a Multiprotein Transmembrane Complex: The Functional SecYEG-SecDF-YajC-YidC Holotranslocon Protein Secretase/Insertase.

Meth. Enzymol. 556:23-49. doi: 10.1016/bs.mie.2014.12.027
Europe PMC | doi

Nguyen-Huynh NT, Sharov G, Potel C, Fichter P, Trowitzsch S, Berger I, Lamour V, Schultz P, Potier N, Leize-Wagner E. (2015)

Chemical cross-linking and mass spectrometry to determine the subunit interaction network in a recombinant human SAGA HAT subcomplex.

Protein Sci. doi: 10.1002/pro.2676
Europe PMC | doi

von Loeffelholz O, Jiang Q, Ariosa A, Karuppasamy M, Huard K, Berger I, Shan SO, Schaffitzel C. (2015)

Ribosome-SRP-FtsY cotranslational targeting complex in the closed state.

Proc. Natl. Acad. Sci. U.S.A. 112(13):3943-3948. doi: 10.1073/pnas.1424453112
Europe PMC | doi

Trowitzsch S, Viola C, Scheer E, Conic S, Chavant V, Fournier M, Papai G, Ebong IO, Schaffitzel C, Zou J, Haffke M, Rappsilber J, Robinson CV, Schultz P, Tora L, Berger I. (2015)

Cytoplasmic TAF2-TAF8-TAF10 complex provides evidence for nuclear holo-TFIID assembly from preformed submodules.

Nat Commun 6:6011. doi: 10.1038/ncomms7011
Europe PMC | doi

Abdulrahman W, Radu L, Garzoni F, Kolesnikova O, Gupta K, Osz-Papai J, Berger I, Poterszman A. (2015)

The production of multiprotein complexes in insect cells using the baculovirus expression system.

Methods Mol. Biol. 1261:91-114. doi: 10.1007/978-1-4939-2230-7_5
Europe PMC | doi

Haffke M, Marek M, Pelosse M, Diebold ML, Schlattner U, Berger I, Romier C. (2015)

Characterization and Production of Protein Complexes by Co-expression in Escherichia coli.

Methods Mol. Biol. 1261:63-89. doi: 10.1007/978-1-4939-2230-7_4
Europe PMC | doi


Reich S, Guilligay D, Pflug A, Malet H, Berger I, Crépin T, Hart D, Lunardi T, Nanao M, Ruigrok RW, Cusack S. (2014)

Structural insight into cap-snatching and RNA synthesis by influenza polymerase.

Nature 516:361-366. doi: 10.1038/nature14009
Europe PMC | doi

Thimiri Govinda Raj DB, Garzoni F, Gavin AC, Gibson T, Berger I. (2014)

FEBS-EMBO Concurrent Session (lecture): CS III-6-3 SynBac: designer minimal baculovirus genome for drug discovery.

Paper presented at the FEBS EMBO 2014 Conference, Paris, France, 30 August-4 September 2014.FEBS J. 281(S1):9-55. doi: 10.1111/febs.12917

Schulze RJ, Komar J, Botte M, Allen WJ, Whitehouse S, Gold VA, Lycklama A Nijeholt JA, Huard K, Berger I, Schaffitzel C, Collinson I. (2014)

Membrane protein insertion and proton-motive-force-dependent secretion through the bacterial holo-translocon SecYEG-SecDF-YajC-YidC

Proc. Natl. Acad. Sci. U.S.A. 111(13):4844-4849. doi: 10.1073/pnas.1315901111
Europe PMC | doi

Kandiah E, Trowitzsch S, Gupta K, Haffke M, Berger I. (2014)

More pieces to the puzzle: recent structural insights into class II transcription initiation

Curr. Opin. Struct. Biol. 24C:91-97. doi: 10.1016/j.sbi.2013.12.005
Europe PMC | doi

Nie Y, Bellon-Echeverria I, Trowitzsch S, Bieniossek C, Berger I. (2014)

Multiprotein complex production in insect cells by using polyproteins

Methods Mol. Biol. 1091:131-141. doi: 10.1007/978-1-62703-691-7_8
Europe PMC | doi

Raj DB, Vijayachandran LS, Berger I. (2014)

OmniBac: universal multigene transfer plasmids for baculovirus expression vector systems

Methods Mol. Biol. 1091:123-130. doi: 10.1007/978-1-62703-691-7_7
Europe PMC | doi


Berger I, Chaillet M, Garzoni F, Yau-Rose S, Zoro B. (2013)

High-Throughput Screening of Multiple Protein Complexes

AM LAB 45(8):32-35.

Barford D, Takagi Y, Schultz P, Berger I. (2013)

Baculovirus expression: tackling the complexity challenge

Curr. Opin. Struct. Biol. 23(3):357-364. doi: 10.1016/j.sbi.2013.03.009
Europe PMC | doi

Berger I, Mayr LM. (2013)

Protein production for structural biology: new solutions to new challenges

Curr. Opin. Struct. Biol. 23(3):317-318. doi: 10.1016/j.sbi.2013.05.004
Europe PMC | doi

von Loeffelholz O, Knoops K, Ariosa A, Zhang X, Karuppasamy M, Huard K, Schoehn G, Berger I, Shan SO, Schaffitzel C. (2013)

Structural basis of signal sequence surveillance and selection by the SRP-FtsY complex

Nat. Struct. Mol. Biol. 20(5):604-610. doi: 10.1038/nsmb.2546
Europe PMC | doi

Berger I, Garzoni F, Chaillet M, Haffke M, Gupta K, Aubert A. (2013)

The multiBac protein complex production platform at the EMBL

J Vis Exp (77):e50159. doi: 10.3791/50159
Europe PMC | doi

Bieniossek C, Papai G, Schaffitzel C, Garzoni F, Chaillet M, Scheer E, Papadopoulos P, Tora L, Schultz P, Berger I. (2013)

The architecture of human general transcription factor TFIID core complex

Nature 493(7434):699-702. doi: 10.1038/nature11791
Europe PMC | doi

Haffke M, Viola C, Nie Y, Berger I. (2013)

Tandem recombineering by SLIC cloning and Cre-LoxP fusion to generate multigene expression constructs for protein complex research

Methods Mol. Biol. 1073:131-140. doi: 10.1007/978-1-62703-625-2_11
Europe PMC | doi

Palmberger D, Klausberger M, Berger I, Grabherr R. (2013)

MultiBac turns sweet

Bioengineered 4(2):78-83. doi: 10.4161/bioe.22327
Europe PMC | doi

Vijayachandran LS, Thimiri Govinda Raj DB, Edelweiss E, Gupta K, Maier J, Gordeliy V, Fitzgerald DJ, Berger I. (2013)

Gene gymnastics: Synthetic biology for baculovirus expression vector system engineering

Bioengineered 4(5):279-287. doi: 10.4161/bioe.22966
Europe PMC | doi


Trowitzsch S, Palmberger D, Fitzgerald D, Takagi Y, Berger I. (2012)

MultiBac complexomics

Expert Rev Proteomics 9(4):363-373. doi: 10.1586/epr.12.32
Europe PMC | doi

Bieniossek C, Imasaki T, Takagi Y, Berger I. (2012)

MultiBac: expanding the research toolbox for multiprotein complexes

Trends Biochem. Sci. 37(2):49-57. doi: 10.1016/j.tibs.2011.10.005
Europe PMC | doi

Palmberger D, Wilson IB, Berger I, Grabherr R, Rendic D. (2012)

SweetBac: a new approach for the production of mammalianised glycoproteins in insect cells

PLoS ONE 7(4):e34226. doi: 10.1371/journal.pone.0034226
Europe PMC | doi


Trowitzsch S, Klumpp M, Thoma R, Carralot JP, Berger I. (2011)

Light it up: highly efficient multigene delivery in mammalian cells

Bioessays 33(12):946-955. doi: 10.1002/bies.201100109
Europe PMC | doi

Berger I, Blanco AG, Boelens R, Cavarelli J, Coll M, Folkers GE, Nie Y, Pogenberg V, Schultz P, Wilmanns M, Moras D, Poterszman A. (2011)

Structural insights into transcription complexes

J. Struct. Biol. 175(2):135-146. doi: 10.1016/j.jsb.2011.04.015
Europe PMC | doi

Vijayachandran LS, Viola C, Garzoni F, Trowitzsch S, Bieniossek C, Chaillet M, Schaffitzel C, Busso D, Romier C, Poterszman A, Richmond TJ, Berger I. (2011)

Robots, pipelines, polyproteins: enabling multiprotein expression in prokaryotic and eukaryotic cells

J. Struct. Biol. 175(2):198-208. doi: 10.1016/j.jsb.2011.03.007
Europe PMC | doi

Imasaki T, Calero G, Cai G, Tsai KL, Yamada K, Cardelli F, Erdjument-Bromage H, Tempst P, Berger I, Kornberg GL, Asturias FJ, Kornberg RD, Takagi Y. (2011)

Architecture of the Mediator head module

Nature 475(7355):240-243. doi: 10.1038/nature10162
Europe PMC | doi

Sahlmüller MC, Strating JR, Beck R, Eckert P, Popoff V, Haag M, Hellwig A, Berger I, Brügger B, Wieland FT. (2011)

Recombinant heptameric coatomer complexes: novel tools to study isoform-specific functions

Traffic 12(6):682-692. doi: 10.1111/j.1600-0854.2011.01177.x
Europe PMC | doi

Vijayachandran LS, Viola C, Berger I. (2011)

Baculovirus expression strategies for protein complex production

In: (various eds.), Encyclopedia of Life Sciences (eLS). John Wiley & Sons, Ltd.doi: 10.1002/9780470015902.a0023180
Europe PMC | doi


Trowitzsch S, Bieniossek C, Nie Y, Garzoni F, Berger I. (2010)

New baculovirus expression tools for recombinant protein complex production

J. Struct. Biol. 172(1):45-54. doi: 10.1016/j.jsb.2010.02.010
Europe PMC | doi

Kriz A, Schmid K, Baumgartner N, Ziegler U, Berger I, Ballmer-Hofer K, Berger P. (2010)

A plasmid-based multigene expression system for mammalian cells

Nat Commun 1:120. doi: 10.1038/ncomms1120
Europe PMC | doi


Nie Y, Viola C, Bieniossek C, Trowitzsch S, Vijay-Achandran LS, Chaillet M, Garzoni F, Berger I. (2009)

Getting a grip on complexes

Curr. Genomics 10(8):558-572. doi: 10.2174/138920209789503923
Europe PMC | doi

Bieniossek C, Nie Y, Frey D, Olieric N, Schaffitzel C, Collinson I, Romier C, Berger P, Richmond TJ, Steinmetz MO, Berger I. (2009)

Automated unrestricted multigene recombineering for multiprotein complex production

Nat. Methods 6(6):447-450. doi: 10.1038/nmeth.1326
Europe PMC | doi

Nie Y, Bieniossek C, Frey D, Olieric N, Schaffitzel C. (2009)

ACEMBLing multigene expression vectors by recombineering

Protocol Exchange doi: 10.1038/nprot.2009.104
Europe PMC | doi

Bieniossek C, Berger I. (2009)

Towards eukaryotic structural complexomics

J. Struct. Funct. Genomics 10(1):37-46. doi: 10.1007/s10969-008-9047-6
Europe PMC | doi


Bieniossek C, Richmond TJ, Berger I. (2008)

MultiBac: multigene baculovirus-based eukaryotic protein complex production

Curr Protoc Protein Sci Chapter 5:Unit 5.20. doi: 10.1002/0471140864.ps0520s51
Europe PMC | doi


Fitzgerald DJ, Schaffitzel C, Berger P, Wellinger R, Bieniossek C, Richmond TJ, Berger I. (2007)

Multiprotein expression strategy for structural biology of eukaryotic complexes

Structure 15(3):275-279. doi: 10.1016/j.str.2007.01.016
Europe PMC | doi

Berger I, Schaffitzel C, Bieniossek C. (2007)

Nucleic Acid Science – The Excitement of Discovery: Annual Symposium of the Chemical Society Zurich CGZ, Zurich, October 26, 2007

Chimia (Aarau) 61(12):837-841.
Europe PMC


Fitzgerald DJ, Berger P, Schaffitzel C, Yamada K, Richmond TJ, Berger I. (2006)

Protein complex expression by using multigene baculoviral vectors

Nat. Methods 3(12):1021-1032. doi: 10.1038/nmeth983
Europe PMC | doi

Schaffitzel C, Oswald M, Berger I, Ishikawa T, Abrahams JP, Koerten HK, Koning RI, Ban N. (2006)

Structure of the E. coli signal recognition particle bound to a translating ribosome

Nature 444(7118):503-506. doi: 10.1038/nature05182
Europe PMC | doi

Berger P, Berger I, Schaffitzel C, Tersar K, Volkmer B, Suter U. (2006)

Multi-level regulation of myotubularin-related protein-2 phosphatase activity by myotubularin-related protein-13/set-binding factor-2

Hum. Mol. Genet. 15(4):569-579. doi: 10.1093/hmg/ddi473
Europe PMC | doi


Haeberli P, Berger I, Pallan PS, Egli M. (2005)

Syntheses of 4'-thioribonucleosides and thermodynamic stability and crystal structure of RNA oligomers with incorporated 4'-thiocytosine

Nucleic Acids Res. 33(13):3965-3975. doi: 10.1093/nar/gki704
Europe PMC | doi


Berger I, Fitzgerald DJ, Richmond TJ. (2004)

Baculovirus expression system for heterologous multiprotein complexes

Nat. Biotechnol. 22(12):1583-1587. doi: 10.1038/nbt1036
Europe PMC | doi

Fitzgerald DJ, DeLuca C, Berger I, Gaillard H, Sigrist R, Schimmele K, Richmond TJ. (2004)

Reaction cycle of the yeast Isw2 chromatin remodeling complex

EMBO J. 23(19):3836-3843. doi: 10.1038/sj.emboj.7600364
Europe PMC | doi


Berger P, Schaffitzel C, Berger I, Ban N, Suter U. (2003)

Membrane association of myotubularin-related protein 2 is mediated by a pleckstrin homology-GRAM domain and a coiled-coil dimerization module

Proc. Natl. Acad. Sci. U.S.A. 100(21):12177-12182. doi: 10.1073/pnas.2132732100
Europe PMC | doi

Berger I, Bieniossek C, Schaffitzel C, Hassler M, Santelli E, Richmond TJ. (2003)

Direct interaction of Ca2+/calmodulin inhibits histone deacetylase 5 repressor core binding to myocyte enhancer factor 2

J. Biol. Chem. 278(20):17625-17635. doi: 10.1074/jbc.m301646200
Europe PMC | doi


Schaffitzel C, Berger I, Postberg J, Hanes J, Lipps HJ, Plückthun A. (2001)

In vitro generated antibodies specific for telomeric guanine-quadruplex DNA react with Stylonychia lemnae macronuclei

Proc. Natl. Acad. Sci. U.S.A. 98(15):8572-8577. doi: 10.1073/pnas.141229498
Europe PMC | doi

Spacková N, Berger I, Sponer J. (2001)

Structural dynamics and cation interactions of DNA quadruplex molecules containing mixed guanine/cytosine quartets revealed by large-scale MD simulations

J. Am. Chem. Soc. 123(14):3295-3307. doi: 10.1021/ja002656y
Europe PMC | doi


Berger I, Egli M, Rich A. (1996)

Inter-strand C-H...O hydrogen bonds stabilizing four-stranded intercalated molecules: stereoelectronic effects of O4' in cytosine-rich DNA

Proc. Natl. Acad. Sci. U.S.A. 93(22):12116-12121. doi: 10.1073/pnas.93.22.12116
Europe PMC | doi


Kang C, Chan R, Berger I, Lockshin C, Green L, Gold L, Rich A. (1995)

Crystal structure of the T4 regA translational regulator protein at 1.9 A resolution

Science 268(5214):1170-1173. doi: 10.1126/science.7761833
Europe PMC | doi