Total: 36 publication(s)


Simon B, Huart A, Temmerman K, Vahokoski J, Mertens H, Komadina D, Hoffmann J, Yumerefendi H, Svergun D, Kursula P, Schultz C, McCarthy A, Hart D, Wilmanns M. (2016)

Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites

Structure doi: 10.1016/j.str.2016.03.020

Thierry E, Guilligay D, Kosinski J, Bock T, Gaudon S, Round A, Pflug A, Hengrung N, El Omari K, Baudin F, Hart DJ, Beck M, Cusack S. (2016)

Influenza Polymerase Can Adopt an Alternative Configuration Involving a Radical Repacking of PB2 Domains.

Mol. Cell 61(1):125-137. doi: 10.1016/j.molcel.2015.11.016
Europe PMC | doi


Delaforge E, Milles S, Bouvignies G, Bouvier D, Boivin S, Salvi N, Maurin D, Martel A, Round A, Lemke EA, Jensen MR, Hart D, Blackledge M. (2015)

Large Scale Conformational Dynamics Control H5N1 Influenza Polymerase PB2 Binding to Importin α.

J. Am. Chem. Soc. doi: 10.1021/jacs.5b07765
Europe PMC | doi


Reich S, Guilligay D, Pflug A, Malet H, Berger I, Crépin T, Hart D, Lunardi T, Nanao M, Ruigrok RW, Cusack S. (2014)

Structural insight into cap-snatching and RNA synthesis by influenza polymerase.

Nature 516:361-366. doi: 10.1038/nature14009
Europe PMC | doi

Sukackaite R, Jensen MR, Mas PJ, Blackledge M, Buonomo SB, Hart DJ. (2014)

Structural and Biophysical Characterization of Murine Rif1 C Terminus Reveals High Specificity for DNA Cruciform Structures

J. Biol. Chem. 289(20):13903-13911. doi: 10.1074/jbc.m114.557843
Europe PMC | doi


Hart DJ, Waldo GS. (2013)

Library methods for structural biology of challenging proteins and their complexes

Curr. Opin. Struct. Biol. 23(3):403-408. doi: 10.1016/
Europe PMC | doi


Chatterjee J, Beullens M, Sukackaite R, Qian J, Lesage B, Hart DJ, Bollen M, Köhn M. (2012)

Development of a peptide that selectively activates protein phosphatase-1 in living cells

Angew. Chem. Int. Ed. Engl. 51(40):10054-10059. doi: 10.1002/anie.201204308
Europe PMC | doi

Levdikov VM, Blagova EV, Rawlings AE, Jameson K, Tunaley J, Hart DJ, Barak I, Wilkinson AJ. (2012)

Structure of the phosphatase domain of the cell fate determinant SpoIIE from Bacillus subtilis

J. Mol. Biol. 415(2):343-358. doi: 10.1016/j.jmb.2011.11.017
Europe PMC | doi

Pumroy RA, Nardozzi JD, Hart DJ, Root MJ, Cingolani G. (2012)

Nucleoporin Nup50 stabilizes closed conformation of armadillo repeat 10 in importin α5

J. Biol. Chem. 287(3):2022-2031. doi: 10.1074/jbc.m111.315838
Europe PMC | doi


Yumerefendi H, Desravines DC, Hart DJ. (2011)

Library-based methods for identification of soluble expression constructs

Methods 55(1):38-43. doi: 10.1016/j.ymeth.2011.06.007
Europe PMC | doi

An Y, Yumerefendi H, Mas PJ, Chesneau A, Hart DJ. (2011)

ORF-selector ESPRIT: a second generation library screen for soluble protein expression employing precise open reading frame selection

J. Struct. Biol. 175(2):189-197. doi: 10.1016/j.jsb.2011.04.004
Europe PMC | doi

Boivin S, Hart DJ. (2011)

Interaction of the influenza A virus polymerase PB2 C-terminal region with importin alpha isoforms provides insights into host adaptation and polymerase assembly

J. Biol. Chem. 286(12):10439-10448. doi: 10.1074/jbc.m110.182964
Europe PMC | doi

An Y, Meresse P, Mas PJ, Hart DJ. (2011)

CoESPRIT: a library-based construct screening method for identification and expression of soluble protein complexes

PLoS ONE 6(2):e16261. doi: 10.1371/journal.pone.0016261
Europe PMC | doi


Rawlings AE, Levdikov VM, Blagova E, Colledge VL, Mas PJ, Tunaley J, Vavrova L, Wilson KS, Barak I, Hart DJ, Wilkinson AJ. (2010)

Expression of soluble, active fragments of the morphogenetic protein SpoIIE from Bacillus subtilis using a library-based construct screen

Protein Eng. Des. Sel. 23(11):817-825. doi: 10.1093/protein/gzq057
Europe PMC | doi

Yumerefendi H, Tarendeau F, Mas PJ, Hart DJ. (2010)

ESPRIT: an automated, library-based method for mapping and soluble expression of protein domains from challenging targets

J. Struct. Biol. 172(1):66-74. doi: 10.1016/j.jsb.2010.02.021
Europe PMC | doi

Boivin S, Cusack S, Ruigrok RW, Hart DJ. (2010)

Influenza A virus polymerase: structural insights into replication and host adaptation mechanisms

J. Biol. Chem. 285(37):28411-28417. doi: 10.1074/jbc.r110.117531
Europe PMC | doi

Nadal M, Mas PJ, Blanco AG, Arnan C, Solà M, Hart DJ, Coll M. (2010)

Structure and inhibition of herpesvirus DNA packaging terminase nuclease domain

Proc. Natl. Acad. Sci. U.S.A. 107(37):16078-16083. doi: 10.1073/pnas.1007144107
Europe PMC | doi

Oger F, Lecorgne A, Sala E, Nardese V, Demay F, Chevance S, Desravines DC, Aleksandrova N, Le Guével R, Lorenzi S, Beccari AR, Barath P, Hart DJ, Bondon A, Carettoni D, Simonneaux G, Salbert G. (2010)

Biological and biophysical properties of the histone deacetylase inhibitor suberoylanilide hydroxamic acid are affected by the presence of short alkyl groups on the phenyl ring

J. Med. Chem. 53(5):1937-1950. doi: 10.1021/jm901561u
Europe PMC | doi

Ruigrok RW, Crépin T, Hart DJ, Cusack S. (2010)

Towards an atomic resolution understanding of the influenza virus replication machinery

Curr. Opin. Struct. Biol. 20(1):104-113. doi: 10.1016/
Europe PMC | doi

Chenavas S, Guilligay D, Cusack S, Hart DJ, Ruigrok RWH, Crépin T. (2010)

Avancées dans l'étude structurale et la compréhension moléculaire du mécanisme de transcription du virus de la grippe [Advances in the structural study and molecular comprehension of the transcription mechanism of influenza virus]

Virologie 14(6):435-445. doi: 10.1684/vir.2010.0328
Europe PMC | doi


Morinière J, Rousseaux S, Steuerwald U, Soler-López M, Curtet S, Vitte AL, Govin J, Gaucher J, Sadoul K, Hart DJ, Krijgsveld J, Khochbin S, Müller CW, Petosa C. (2009)

Cooperative binding of two acetylation marks on a histone tail by a single bromodomain

Nature 461(7264):664-668. doi: 10.1038/nature08397
Europe PMC | doi

Bonneau F, Lenherr ED, Pena V, Hart DJ, Scheffzek K. (2009)

Solubility survey of fragments of the neurofibromatosis type 1 protein neurofibromin

Protein Expr. Purif. 65(1):30-37. doi: 10.1016/j.pep.2008.12.001
Europe PMC | doi

Angelini A, Tosi T, Mas P, Acajjaoui S, Zanotti G, Terradot L, Hart DJ. (2009)

Expression of Helicobacter pylori CagA domains by library-based construct screening

FEBS J. 276(3):816-824. doi: 10.1111/j.1742-4658.2008.06826.x
Europe PMC | doi


Chesneau A, Yumerefendi H, Hart DJ. (2008)

The Impact of Protein Expression Methodologies on Structural Proteomics

In: Sussmann JL, Silman I (eds.), Structural Proteomics and Its Impact on the Life Sciences. WORLD SCIENTIFIC. pp 207-231.doi: 10.1142/9789812772053_0009
Europe PMC | doi

Guilligay D, Tarendeau F, Resa-Infante P, Coloma R, Crepin T, Sehr P, Lewis J, Ruigrok RW, Ortin J, Hart DJ, Cusack S. (2008)

The structural basis for cap binding by influenza virus polymerase subunit PB2

Nat. Struct. Mol. Biol. 15(5):500-506. doi: 10.1038/nsmb.1421
Europe PMC | doi

Tarendeau F, Crepin T, Guilligay D, Ruigrok RW, Cusack S, Hart DJ. (2008)

Host determinant residue lysine 627 lies on the surface of a discrete, folded domain of influenza virus polymerase PB2 subunit

PLoS Pathog. 4(8):e1000136. doi: 10.1371/journal.ppat.1000136
Europe PMC | doi


Tarendeau F, Boudet J, Guilligay D, Mas PJ, Bougault CM, Boulo S, Baudin F, Ruigrok RW, Daigle N, Ellenberg J, Cusack S, Simorre JP, Hart DJ. (2007)

Structure and nuclear import function of the C-terminal domain of influenza virus polymerase PB2 subunit

Nat. Struct. Mol. Biol. 14(3):229-233. doi: 10.1038/nsmb1212
Europe PMC | doi


Alzari PM, Berglund H, Berrow NS, Blagova E, Busso D, Cambillau C, Campanacci V, Christodoulou E, Eiler S, Fogg MJ, Folkers G, Geerlof A, Hart D, Haouz A, Herman MD, Macieira S, Nordlund P, Perrakis A, Quevillon-Cheruel S, Tarandeau F, van Tilbeurgh H, Unger T, Luna-Vargas MP, Velarde M, Willmanns M, Owens RJ. (2006)

Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE

Acta Crystallogr. D Biol. Crystallogr. 62(Pt 10):1103-1113. doi: 10.1107/s0907444906029775
Europe PMC | doi

Hart DJ, Tarendeau F. (2006)

Combinatorial library approaches for improving soluble protein expression in Escherichia coli

Acta Crystallogr. D Biol. Crystallogr. 62(Pt 1):19-26. doi: 10.1107/s0907444905036097
Europe PMC | doi


Yoshida H, Hensgens CM, van der Laan JM, Sutherland JD, Hart DJ, Dijkstra BW. (2005)

An approach to prevent aggregation during the purification and crystallization of wild type acyl coenzyme A: isopenicillin N acyltransferase from Penicillium chrysogenum

Protein Expr. Purif. 41(1):61-67. doi: 10.1016/j.pep.2005.02.007
Europe PMC | doi

Blackburn JM, Hart DJ. (2005)

Fabrication of protein function microarrays for systems-oriented proteomic analysis

Methods Mol. Biol. 310:197-216. doi: 10.1007/978-1-59259-948-6_14
Europe PMC | doi


Boutell JM, Hart DJ, Godber BL, Kozlowski RZ, Blackburn JM. (2004)

Functional protein microarrays for parallel characterisation of p53 mutants

Proteomics 4(7):1950-1958. doi: 10.1002/pmic.200300722
Europe PMC | doi

de Lumley M, Hart DJ, Cooper MA, Symeonides S, Blackburn JM. (2004)

A biophysical characterisation of factors controlling dimerisation and selectivity in the NF-kappaB and NFAT families

J. Mol. Biol. 339(5):1059-1075. doi: 10.1016/j.jmb.2004.03.083
Europe PMC | doi


Speight RE, Hart DJ, Blackburn JM. (2002)

Distamycin A affects the stability of NF-kappaB p50-DNA complexes in a sequence-dependent manner

J. Mol. Recognit. 15(1):19-26. doi: 10.1002/jmr.556
Europe PMC | doi


Hart DJ, Speight RE, Sutherland JD, Blackburn JM. (2001)

Analysis of the NF-kappaB p50 dimer interface by diversity screening

J. Mol. Biol. 310(3):563-575. doi: 10.1006/jmbi.2001.4724
Europe PMC | doi


Hart DJ, Speight RE, Cooper MA, Sutherland JD, Blackburn JM. (1999)

The salt dependence of DNA recognition by NF-kappaB p50: a detailed kinetic analysis of the effects on affinityand specificity

Nucleic Acids Res. 27(4):1063-1069. doi: 10.1093/nar/27.4.1063
Europe PMC | doi