BM14 is bending magnet 14 beamline at the ESRF, optimised for exploiting anomalous scattering methods MAD/SAD in macromolecular structure determination. BM14 has an optical design suited to the rapid and accurate energy change between 7 and 17 kev, which covers most of the commonly used absorption edges of heavy atoms. BM14 provides beamtime and support to scientists from India (via Department of Biotechnology (DBT) funding) and Europe (via European Synchrotron Radiation Facility (ESRF) funding and the BioStruct-X European Commission Programme) on the basis of peer-reviewed beamtime proposals.
Ballandras-Colas A et al. (2016)
Cryo-EM reveals a novel octameric integrase structure for betaretroviral intasome function.
Nature 530, 358–361, doi:10.1038/nature16955
- Energy range 7-17 keV
- Sulphur SAD
- EMBL-ESRF Robotic SPINE Compatible Sample Changer (SC3)
- MD2 Microdiffractometer (EMBL-Maatel)
- Mini-Kappa goniometer for crystal reorientation
- MarCCD detector (active area of 225 x 225 mm)
- Fluorescence Detector (Roentec)
- Humidity Control Device for crystal dehydration (HC1)
- Remotely Accessible Beamline
- In Situ data collection – Crystal Direct
- Nozzle Changer (NC1) system (Arinax)
BM14 offers excellent facilities for users, including sample preparation laboratory and computing resources. A dedicated team of scientists assists users before, during and after the experiments to ensure optimal experimental conditions.
NOTE: For all users publishing data collected on BM14 please do add the following sentence in the acknowledgments:
We thank the EMBL staff Dr Hassan Belrhali or/and Dr. Babu A. Manjasetty for providing support on the beamline and EMBL-DBT for providing access to the BM14 beamline at the ESRF.